Genome-wide association analysis of stripe rust resistance loci in wheat accessions from southwestern China
Genome-wide association analysis of stripe rust resistance loci in wheat accessions from southwestern China
复制标题
中国西南小麦种质中条锈病抗性位点的全基因组关联分析
DOI：
10.1007/s13353-019-00533-8
复制
发表时间：
2020-02-01
影响因子：
2.4
通讯作者：
Zhang, Liyi
中科院分区：
文献类型：
--
作者：
Cheng, Bin;Gao, Xu;Zhang, Liyi
小麦条锈病可造成严重的产量损失，遗传抗性是控制该病的最有效途径。确定小麦条锈菌的基因组区域。为了研究中国西南地区冬小麦抗条锈病（Pst）的遗传多样性，并筛选这些地区的DNA标记，利用90 K小麦SNP芯片中的单核苷酸多态性（SNP）标记对120份中国冬小麦材料进行了全基因组关联分析。在田间和温室试验中，共有16个SNP位点与小麦条锈病显著相关。其中，2014年在绵阳的一个地点鉴定了染色体1B、4A和6A上的三个独特的SNP，自2009年以来最流行的小麦条锈病小种是V26（G22-9，G22-14）。这表明这三个SNP位点与新的抗V26小种的数量性状位点（QTL）/基因连锁。对Pstis具有免疫性的种质是小麦抗条锈育种的良好资源，进一步验证后，与抗病QTL/基因紧密连锁的SNPs可转化为用户友好的标记，便于标记辅助选择，提高小麦抗条锈性。
Wheat stripe rust can cause considerable yield losses, and genetic resistance is the most effective approach for controlling the disease. To identify the genomic regions responsible forPuccinia striiformisf. sp.tritici(Pst) resistance in a set of winter wheat strains mainly from southwestern China, and to identify DNA markers in these regions, we carried out a genome-wide association study (GWAS) of 120 China winter wheat accessions using single nucleotide polymorphism (SNP) markers from 90K wheat SNP arrays. In total, 16 SNP loci were significantly associated with wheat stripe rust in field and greenhouse trials. Of these, three distinctive SNPs on chromosomes 1B, 4A, and 6A were identified at a site in Mianyang in 2014, where the most prevalent wheat stripe rust races since 2009 have been V26 (G22-9, G22-14). This suggests that the three SNP loci were linked to the new quantitative trait loci (QTL)/genes resistant to the V26 races. Germplasm with immunity toPstis a good source of stripe rust resistance for breeding, and after further validation, SNPs closely linked to resistance QTLs/genes could be converted into user-friendly markers and facilitate marker-assisted selection to improve wheat stripe rust resistance.