Peroxynitrite formation by diesel exhaust particles in alveolar cells: Links to pulmonary inflammation
Peroxynitrite formation by diesel exhaust particles in alveolar cells: Links to pulmonary inflammation
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DOI：
10.1016/s1382-6689(00)00053-3
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发表时间：
2000-12-01
影响因子：
4.3
通讯作者：
Tomita, T
中科院分区：
文献类型：
--
作者：
Ito, T;Ikeda, M;Tomita, T
柴油机排气颗粒物（DEP）被认为是肺部炎症的致病物质。由于过氧亚硝酸盐最近与炎症和细胞毒性有关，因此验证了滴注DEP诱导肺中迁移的细胞中过氧亚硝酸盐形成的假设。给大鼠气管内滴入DEP混悬液（2 mg/0.5ml/kg），24 h后处死。通过支气管肺泡灌洗收集肺泡细胞。DEP暴露后肺泡细胞数量增加了两倍以上，主要是由于中性粒细胞的大量增加。实验结果表明，DEP可诱导肺泡细胞产生过氧亚硝酸盐（N（-）（G）硝基-L-精氨酸甲酯和超氧化物歧化酶的化学发光），12-O-十四酰佛波醇13-乙酸酯刺激可增强过氧亚硝酸盐的形成，DEP还可诱导肺泡细胞诱导型NO合成酶mRNA的表达。但在对照组细胞中未检测到过氧亚硝酸盐。这些结果表明DEP暴露导致迁移细胞中过氧亚硝酸盐的形成，这导致肺部炎症。(C)2000 Elsevier Science B. V.保留所有权利。
Diesel exhaust particles (DEP) are assumed to be a causal substance for pulmonary inflammation. As peroxynitrite is recently implicated in inflammation and cytotoxity, the hypothesis was tested that instillation of DEP induces formation of peroxynitrite in cells migrated in lung. Rats were intratracheally instilled with DEP suspension (2 mg/0.5 ml/kg) and killed 24 h later. Alveolar cells were collected by broncho-alveolar lavage. Population of alveolar cells increased more than twice by DEP exposure, mainly due to a large increase of neutrophils. Peroxynitrite formation (N(-)(G)nitro-L-arginine methylester and superoxide dismutase inhibitable chemiluminescence) was detected in alveolar cells from treated rats, and 12-O-tetradecanoylphorbol 13-acetate-stimulation enhanced it. In addition, DEP induced expression of inducible NO synthase mRNA in these cells. But peroxynitrite was not detectable in cells from control. These results indicate that DEP exposure results in peroxynitrite formation in migrated cells, which leads to pulmonary inflammation. (C) 2000 Elsevier Science B.V. All rights reserved.