Adiponectin enhances in vitro development of swine embryos
Adiponectin enhances in vitro development of swine embryos
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DOI：
10.1016/j.domaniend.2008.05.007
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发表时间：
2008-08-01
影响因子：
2.1
通讯作者：
Bordignon, Vilceu
中科院分区：
文献类型：
--
作者：
Chappaz, Eugenie;Albornoz, Marcelo S.;Bordignon, Vilceu
最近的研究表明，来自脂肪组织的因素影响和调节生殖系统。现在已知瘦素和瘦素等激素调节几种生殖过程。脂联素是脂肪组织分泌的最丰富的蛋白质，其循环浓度与肥胖和体重指数呈负相关。关于脂联素参与生殖的研究知之甚少。以猪卵母细胞为材料，研究了重组脂联素对猪卵母细胞减数分裂成熟和早期胚胎体外发育的影响。脂联素受体AdipoR 1和AdipoR 2在猪小卵泡和大卵泡的卵母细胞和卵丘细胞中均有表达。AdipoR 1和AdipoR 2均免疫定位于卵丘-卵母细胞复合物（COCs）、卵母细胞和早期发育的胚胎。在卵母细胞成熟培养液中培养46 h后，脂联素可显著降低大卵泡（3-6 mm）成熟卵母细胞的比例，但对小卵泡（< 3 mm）成熟卵母细胞的比例无影响。从在脂联素和丝裂原活化蛋白激酶（MAPK）通路抑制剂MEK 1（PD 98059）、MEK 1/2（U 0126）和p38 MAPK（SB 203580）存在下成熟的卵母细胞的研究中得出结论，脂联素通过p38 MAPK通路促进卵母细胞成熟。最后，在脂联素存在下培养的胚胎达到了胚胎发育到囊胚阶段的上级速率。这些结果表明，脂联素对猪卵母细胞的减数分裂成熟和体外胚胎发育具有积极的作用，并表明这种脂肪因子在哺乳动物早期发育中的生理作用。(c)2008年爱思唯尔公司All rights reserved.
Recent studies have shown that factors from adipose tissue influence and regulate the reproductive system. Hormones such as leptin and resistin are now known to regulate several reproductive processes. Adiponectin is the most abundant protein secreted by adipose tissue, and its circulating concentration is inversely related to adiposity and body mass index. Little is known about the involvement of adiponectin in reproduction. In the present study, the effect of recombinant adiponectin on the meiotic maturation and early embryo development in vitro was investigated, using porcine oocytes. Adiponectin receptors, AdipoR1 and AdipoR2, were found to be expressed in porcine oocytcs and cumulus cells of both small and large follicles. Both AdipoR1 and AdipoR2 were immunolocalized to cumulus-oocyte complexes (COCs), oocytes, and early developing embryos. When included in oocyte maturation medium for 46 h, adiponectin significantly decreased the frequency of meiotic immature oocytes derived from large follicles (3-6 mm) but not from small follicles (< 3 mm). From studies of oocytes matured in the presence of adiponectin and mitogen-activated protein kinase (MAPK) pathway inhibitors MEK1 (PD98059), MEK1/2 (U0126), and p38MAPK (SB203580) it was concluded that adiponectin enhances oocyte maturation thought the p38MAPK pathway. Finally, a superior rate of embryo development to the blastocyst stage was achieved by embryos cultured in the presence of adiponectin. These results indicate that adiponectin has a positive effect on the meiotic maturation and in vitro embryo development of porcine oocytes and suggests a physiological role for this adipokine in early development in mammals. (c) 2008 Elsevier Inc. All rights reserved.