Comparison of Co-housing and Littermate Methods for Microbiota Standardization in Mouse Models
Comparison of Co-housing and Littermate Methods for Microbiota Standardization in Mouse Models
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DOI：
10.1016/j.celrep.2019.04.023
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发表时间：
2019-05-07
期刊：
影响因子：
8.8
通讯作者：
Philpott, Dana J.
中科院分区：
文献类型：
--
作者：
Robertson, Susan J.;Lemire, Paul;Philpott, Dana J.
肠道微生物群是广泛影响生理学的基本因素。因此，使用转基因动物的研究应旨在限制菌株之间微生物群变异的混杂效应。在这里，我们报告了共同饲养与F2代同窝仔对肠道微生物群的影响，这是两种常用的标准化动物模型微生物群的技术。我们的研究结果表明，虽然粪便微生物群通过延长共同饲养而部分正常化，但与近端结肠和末端回肠相关的粘膜群落保持稳定和独特。相比之下，菌株互交以产生F2同窝仔允许粪便、结肠和回肠取样位置中的稳健微生物群标准化。利用互惠互交的P1父母，我们确定不对称的F2社区结构所造成的孕产妇传播，特别是Verrucomicrobiaceae。因此，来自单向P1杂交的F2同窝动物应用作标准方法，以最大限度地减少基因型-表型研究中微生物群的影响。
The intestinal microbiota is a fundamental factor that broadly influences physiology. Thus, studies using transgenic animals should be designed to limit the confounding effects of microbiota variation between strains. Here, we report the impact on intestinal microbiota of co-housed versus F2-generation littermates, two commonly used techniques to standardize microbiota in animal models. Our results establish that while fecal microbiota is partially normalized by extended co-housing, mucosal communities associated with the proximal colon and terminal ileum remain stable and distinct. In contrast, strain inter-crossing to generate F2 littermates allows robust microbiota standardization in fecal, colon, and ileum sampling locations. Using reciprocal inter-crosses of P1 parents, we identify dissymmetry in F2 community structures caused by maternal transmission, in particular of the Verrucomicrobiaceae. Thus, F2 littermate animals from a unidirectional P1 cross should be used as a standard method to minimize the influence of the microbiota in genotype-phenotype studies.