Duck hepatitis B virus virion secretion requires a double-stranded DNA genome.
Duck hepatitis B virus virion secretion requires a double-stranded DNA genome.
复制标题
鸭乙型肝炎病毒病毒粒子的分泌需要双链DNA基因组。
DOI：
10.1128/jvi.77.3.2287-2294.2003
复制
发表时间：
2003
影响因子：
5.4
通讯作者：
Hu,Jianming
中科院分区：
文献类型：
--
作者：
Perlman,David;Hu,Jianming
Hepatitis B virus assembly begins with the packaging of an RNA pregenome into intracellular nucleocapsids, with subsequent reverse transcription within these nucleocapsids converting the RNA into a characteristic, partially double-stranded DNA, which, alone, is found in enveloped extracellular virions as the viral genome. Using a synchronized replication system for the duck hepatitis B virus, together with a stringent two-step assay for virion secretion, we demonstrate that this selective genome secretion results from an intrinsic secretion competence gained only by the nucleocapsids at the late stage of reverse transcription.
