Correlation between rate constant for reduction and redox potential as a basis for systematic investigation of reaction mechanisms of electron transfer proteins.
Correlation between rate constant for reduction and redox potential as a basis for systematic investigation of reaction mechanisms of electron transfer proteins.
复制标题
还原速率常数与氧化还原电位之间的相关性作为系统研究电子转移蛋白反应机制的基础。
DOI：
10.1073/pnas.80.22.6740
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发表时间：
1983
影响因子：
11.1
通讯作者：
Tollin,G
中科院分区：
文献类型：
--
作者：
Meyer,TE;Przysiecki,CT;Watkins,JA;Bhattacharyya,A;Simondsen,RP;Cusanovich,MA;Tollin,G
用激光闪光光解技术测定了光还原黄素还原约40种电子转移蛋白的速率常数。一系列的12个同源的细胞色素和10个同源的高氧化还原电位铁氧化还原蛋白（HiPPs）的数据是在极好的协议与半经验方程有关的速率常数和热力学氧化还原电位，已被证明适用于非生物电子转移系统。这些相关性允许建立相对反应性内的结构同源类的生物氧化还原蛋白，包括细胞色素和HiPIPs，和各种非同源血红素，铁硫，铜，和黄素含蛋白。一个定性的对应关系示出存在于这样的相对反应性和溶剂暴露的氧化还原中心在一个特定的结构类的程度之间。这些结果的影响被认为是，它的结论是，自由能的关系提供了一个良好的基础，系统分析的反应机制的电子转移蛋白质。
Rate constants for the reduction of approximately 40 electron transfer proteins by photoreduced flavins have been determined by laser flash photolysis techniques. The data for a series of 12 homologous cytochromes and 10 homologous high redox potential ferredoxins (HiPIPs) are in excellent agreement with semi-empirical equations relating rate constant and thermodynamic redox potential that have proven applicable to nonbiological electron transfer systems. These correlations allow the establishment of relative reactivities within structurally homologous classes of biological oxidation-reduction proteins, including cytochromes and HiPIPs, and a variety of nonhomologous heme-, iron-sulfur-, copper-, and flavin-containing proteins. A qualitative correspondence is shown to exist between such relative reactivity and the extent of solvent exposure of the redox centers in a particular structural class. The implications of these results are considered, and it is concluded that free energy relationships provide a sound basis for systematic analysis of reaction mechanisms of electron transfer proteins.
c型细胞色素的新观点。
影响因子：
--
作者：
Terrance E. Meyer;Martin D. Kamen
通讯作者：
Martin D. Kamen