Specific 14C labeling of sialic acid and N-acetylhexosamine residues of glycosphingolipids after hydrazinolysis.
Specific 14C labeling of sialic acid and N-acetylhexosamine residues of glycosphingolipids after hydrazinolysis.
复制标题
肼解后鞘糖脂唾液酸和 N-乙酰己糖胺残基的特异性 14C 标记。
DOI：
10.1016/0003-2697(82)90331-1
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发表时间：
1982
影响因子：
2.9
通讯作者：
Basu,S
中科院分区：
文献类型：
--
作者：
Higashi,H;Basu,S
唾液酸和糖鞘脂的氨基糖部分被肼水解n -去乙酰化（或n -去糖酰化），而神经酰胺残留物没有大量（5%）n -去酰化。肼解后各种鞘糖脂的总回收率为：球苷，52%；neolactopentaosylceramide, 46%;sialyl-lactosylceramide, 50%;N-glycolylneuraminyl-neolactotetraosylceramide, 36%;GM1占30%。将n -去乙酰化的鞘脂与[1-14C]乙酸酐定量（96%）重新n -乙酰化，得到高比活性的[乙酰- 14c]鞘脂。
The sialic acid and the amino sugar moieties of glycosphinoglipids were N-deacetylated (or N-deglycolylated) by hydrazinolysis without considerable (5%) N-deacylation of ceramide residue. The total recoveries of various glycosphingolipids after hydrazinolysis were globoside, 52%; neolactopentaosylceramide, 46%; sialyl-lactosylceramide, 50%; N-glycolylneuraminyl-neolactotetraosylceramide, 36%; and GM1, 30%. The N-deacetylated glycosphingolipids were re-N-acetylated quantitatively (96%) with [1-14C]acetic anhydride to obtain [acetyl-14C]glycosphingolipids of high specific activity.