Nanofibers Made of Globular Proteins
Nanofibers Made of Globular Proteins
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DOI：
10.1021/bm8005243
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发表时间：
2008-10-01
影响因子：
6.2
通讯作者：
Zussman, Eyal
中科院分区：
文献类型：
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作者：
Dror, Yael;Ziv, Tamar;Zussman, Eyal
在不使用化学交联剂的情况下，直接从牛血清白蛋白溶液中静电纺丝制备了完全由模型球状蛋白即牛血清白蛋白（BSA）组成的强纳米纤维。通过控制蛋白质构象、蛋白质聚集和分子内/分子间二硫键交换来控制所制备的纳米纤维的可纺性和机械性能。通过这种方法，一种低粘度的球状蛋白溶液可以被修饰成类似聚合物的可纺溶液，并且很容易纺成纤维，其机械性能与由纤维蛋白制成的天然纤维一样好。我们在这里证明了新形成的二硫键（分子内/分子间）在纳米纤维的形成和提供优异的机械性能方面都起着主导作用。我们将蛋白质工程化成生物相容性纤维结构的方法可广泛用于生物医学应用，如缝合、伤口敷料和伤口闭合。
Strong nanofibers composed entirely of a model globular protein, namely, bovine serum albumin (BSA), were produced by electrospinning directly from a BSA solution without the use of chemical cross-linkers. Control of the spinnability and the mechanical properties of the produced nanofibers was achieved by manipulating the protein conformation, protein aggregation, and intra/intermolecular disulfide bonds exchange. In this manner, a low-viscosity globular protein solution could be modified into a polymer-like spinnable solution and easily spun into fibers whose mechanical properties were as good as those of natural fibers made of fibrous protein. We demonstrate here that newly formed disulfide bonds (intra/intermolecular) have a dominant role in both the formation of the nanofibers and in providing them with superior mechanical properties. Our approach to engineer proteins into biocompatible fibrous structures may be used in a wide range of biomedical applications such as suturing, wound dressing, and wound closure.