Dielectrophoretic cell isolation in microfluidics channels for high-throughput biomedical applications
Dielectrophoretic cell isolation in microfluidics channels for high-throughput biomedical applications
复制标题
用于高通量生物医学应用的微流体通道中的介电泳细胞分离
DOI：
10.1109/eit.2016.7535256
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
K. Nawarathna
中科院分区：
文献类型：
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作者：
L. Velmanickam;K. Nawarathna
从血液、血清或尿液等生物样品中分离靶细胞而不被其他细胞污染或高通量不丢失靶细胞是开发有效疗法的起点。目前可用的细胞分离技术需要对目标细胞进行额外标记，然后进行漫长的分离过程。这些过程将导致靶细胞污染和降解。为了解决这个问题，提出了介电泳，因为它提供无标记细胞分离。在介电泳细胞分离中，作用于细胞的介电泳力决定了分离的细胞样品的纯度和通量。由于电场梯度是介电泳力的一部分，因此设计能够产生高电场梯度的电极非常重要。在这项工作中，我们提出了可以产生高电场梯度的电极。然后，我们使用有限元软件计算电场梯度并制造电极。最后，我们生产了一种可用于细胞分离的装置。在本文中，我们介绍了我们设备的概念、设计和原型。
The isolation of target cells from biological samples such as blood, serum or urine without contamination with other cells or without loosing target cells in high-throughput is the starting point of developing effective therapy. Currently available techniques for cell isolation require extra labeling of target cells followed by lengthy isolation processes. These processes will cause to contaminate and degrade target cells. To address this issue, dielectrophoresis is proposed as it provide label free cell isolation. In dielectrophoretic cell isolation, dielectrophoretic force acting on cells determines the purity and the throughput of the isolated cell sample. Since the electric field gradients are part of the dielectrophoresis force, it is important to design electrodes that are capable of generating high electric field gradients. In this work, we have proposed electrodes that can generate high electric field gradients. We then calculated the electric field gradients using finite element software and fabricated the electrodes. Finally, we have produced a device that can be used for cell isolation. In this paper, we have presented the concepts, designs and prototypes of our device.