TAFII mutations disrupt Dorsal activation in the Drosophila embryo.
TAFII mutations disrupt Dorsal activation in the Drosophila embryo.
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TAFII 突变破坏了果蝇胚胎的背侧激活。
DOI：
10.1073/pnas.95.23.13483
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发表时间：
1998
影响因子：
11.1
通讯作者：
Tjian,R
中科院分区：
文献类型：
--
作者：
Zhou,J;Zwicker,J;Szymanski,P;Levine,M;Tjian,R
在这项研究中，我们提出了在果蝇胚胎中背部激活剂与有限数量的TFIID复合体相互作用的证据。在体外转录反应和蛋白质结合分析中，TAFII110和TAFII60亚基参与了背部介导的激活。TAFII110和TAFII60的突变导致d1/+雌性胚胎蜗牛和扭转转录模式的改变。这些结果表明，TAFII有助于活体转录的激活，并支持TFIID亚基可能作为增强子结合蛋白的靶标的假说。
In this study, we present evidence that the Dorsal activator interacts with limiting amounts of the TFIID complex in theDrosophilaembryo.In vitrotranscription reactions and protein binding assays implicate the TAFII110 and TAFII60 subunits of the TFIID complex in contributing to Dorsal-mediated activation. Mutations in TAFII110 and TAFII60 result in altered patterns ofsnailandtwisttranscription in embryos derived fromdl/+ females. These results suggest that TAFIIs contribute to the activation of transcriptionin vivoand support the hypothesis that subunits of TFIID may serve as targets of enhancer binding proteins.