MAPKKK15 gene from shrimp Litopenaeus vannamei is transcribed in larva development stages and contributes to WSSV pathogenesis
MAPKKK15 gene from shrimp Litopenaeus vannamei is transcribed in larva development stages and contributes to WSSV pathogenesis
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DOI：
10.1016/j.aquaculture.2020.736324
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发表时间：
2021-03
期刊：
影响因子：
4.5
通讯作者：
Sheng Wang;Haoyang Li;Peng Zhu;Qihui Fu;Bin Yin;Qinyao Li;Rongjian Chen;Xiewu Jiang;S. Weng;Jianguo He;Chaozheng Li
中科院分区：
文献类型：
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作者：
Sheng Wang;Haoyang Li;Peng Zhu;Qihui Fu;Bin Yin;Qinyao Li;Rongjian Chen;Xiewu Jiang;S. Weng;Jianguo He;Chaozheng Li
MAPKKK15是丝裂原活化蛋白激酶激酶（MAPKKK）家族的成员，在细胞应激触发的多种生物学过程中发挥重要作用。本研究从凡纳滨对虾（plitopenaeus vannamei）中克隆并鉴定了MAPKKK15新的同源基因（命名为LvMAPKKK15）。LvMAPKKK15转录物全长4597 bp，包括4317 bp的开放阅读框（ORF），编码1438个氨基酸的推定蛋白。LvMAPKKK15在发育早期大量表达，在P12期转录水平最高。在成体对虾中，组织分布表明，LvMAPKKK15在眼柄、神经和血细胞中高度表达。此外，驴apkkk15的诱导转录能够应对Poly （I: C）和白斑综合征病毒（WSSV）的挑战。rnai介导的LvMAPKKK15基因敲低导致对虾对WSSV感染的敏感性降低，表现为病毒载量降低，存活率提高，抗病毒因子LvVagos转录水平提高。双荧光素酶报告基因实验进一步支持了这一结果：lvapkkk15能够上调多个WSSV即刻早期（IE）基因的启动子活性，但在体外细胞中抑制LvVago1-5的启动子活性。综上所述，这些结果表明，lvapkkk15在幼虫发育阶段转录，并在WSSV发病过程中发挥作用。
MAPKKK15 is a member of the mitogen-activated protein kinase kinase kinase (MAPKKK) family, which plays an important role in multiple biological progresses triggered by cellular stresses. In this study, a new homolog of MAPKKK15 has been cloned and identified from shrimpLitopenaeus vannamei(named as LvMAPKKK15). The full length of LvMAPKKK15 transcript was 4597 bp, including a 4317 bp open reading frame (ORF) that encoded a putative protein of 1438 amino acids. LvMAPKKK15 was abundantly expressed at early development stages with the highest transcriptional level in P12 stage. In adult shrimp, tissue distribution showed that LvMAPKKK15 was highly expressed in eyestalk, nerve and hemocytes. Besides, the induced transcription of LvMAPKKK15 could respond to the challenges of Poly (I: C) and white spot syndrome virus (WSSV). RNAi-mediated knockdown of LvMAPKKK15 caused shrimp less sensitive to WSSV infection, manifested by lessened virus loads, higher survival rate and higher transcription levels of anti-viral factors LvVagos. The results were further supported by the dual-luciferase reporter assays that the LvMAPKKK15 was able to up-regulate the promoter activities of several WSSV immediate early (IE) genes, but suppress the promoter activities of LvVago1–5 in vitro of cells. Together, these results suggested that LvMAPKKK15 was transcribed in larva development stages and played a role during WSSV pathogenesis.