CONCENTRATION OF ROUS-SARCOMA VIRUS FROM TISSUE-CULTURE FLUIDS WITH POLYETHYLENE-GLYCOL
CONCENTRATION OF ROUS-SARCOMA VIRUS FROM TISSUE-CULTURE FLUIDS WITH POLYETHYLENE-GLYCOL
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DOI：
10.1128/aem.30.3.464-471.1975
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发表时间：
1975-01-01
期刊：
影响因子：
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通讯作者：
RITZI, D
中科院分区：
文献类型：
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作者：
BRONSON, DL;ELLIOTT, AY;RITZI, D
从组织培养液与聚乙二醇，有和没有NaCl或硫酸葡聚糖的劳斯肉瘤病毒的浓度，导致病毒颗粒截留在蛋白质碎片中引起的显着和高度可变的损失。用链霉蛋白酶处理浓缩制剂大大提高了病毒体的回收率。通过向组织培养液中加入8%聚乙二醇和0.4 M NaCl，然后用链霉蛋白酶处理浓缩的病毒制剂，获得病毒颗粒的最大回收率。
Concentration of Rous sarcoma virus from tissue culture fluids with polyethylene glycol, with and without NaCl or dextran sulfate, resulted in significant and highly variable losses caused by entrapment of virus particles in proteinaceous debris. Treatment of concentrated preparations with Pronase greatly enhanced the recovery of virions. Maximum recovery of virus particles was obtained by the addition of 8% polyethylene glycol and 0.4 M NaCl to tissue culture fluids, followed by Pronase treatment of the concentrated virus preparations.