Endocrine disrupting effects of dichlorodiphenyltrichloroethane analogues on gonadotropin hormones in pituitary gonadotrope cells
Endocrine disrupting effects of dichlorodiphenyltrichloroethane analogues on gonadotropin hormones in pituitary gonadotrope cells
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二氯二苯基三氯乙烷类似物对垂体促性腺激素细胞的内分泌干扰作用
DOI：
10.1016/j.etap.2014.04.018
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发表时间：
2014
影响因子：
4.3
通讯作者：
Liu Jing
中科院分区：
文献类型：
--
作者：
Zhou Jinghua;Yang Ye;Xiong Kang;Liu Jing
研究表明，暴露于二氯二苯三氯乙烷（DDT）类似物会导致促卵泡激素（FSH）和促黄体激素（LH）不协调。然而，DDT类似物对促性腺激素基因（FSHβ、LHβ和cga α）表达的影响及其机制尚不清楚，而促性腺激素基因是FSH和LH生物合成的限速步骤。在本研究中，我们评估了p，p ' -DDT,o,p ' -DDT,p,p ' -二氯二苯乙烯（p,p ' -DDE）和甲氧氯（MXC）对促性腺激素基因表达和促性腺激素合成的影响。p、p′-DDT和MXC在10−9 ~ 10−7mol/L的浓度范围内刺激促性腺激素基因表达和激素合成，呈剂量依赖性。细胞外信号调节激酶（ERK）的激活是诱导促性腺激素基因表达和激素合成所必需的，p ' -DDT或MXC暴露。本研究首次发现p、p′-DDT和MXC通过ERK途径调控促性腺激素细胞中促性腺激素基因的表达和激素的合成。
It has been shown that exposure to dichlorodiphenyltrichloroethane (DDT) analogues leads to disharmony of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). However, the effects and mechanisms of DDT analogues on the expression of gonadotropin genes (FSHβ, LHβ and Cgα), which is the rate-limiting step of FSH and LH biosynthesis, remain unknown. In this study, we assessed the effects ofp,p′-DDT,o,p′-DDT,p,p′-dichlorodiphenyldichloroethylene (p,p′-DDE) and methoxychlor (MXC) on gonadotropin genes expression and hormones synthesis in gonadotrope cells.p,p′-DDT and MXC at test concentrations ranging from 10−9to 10−7mol/L, stimulated gonadotropin genes expression and hormones synthesis in a dose-dependent manner. The activation of extracellular signal-regulated kinase (ERK) was required for the induction of gonadotropin genes expression and hormones synthesis byp,p′-DDT or MXC exposure. This study showed for the first time thatp,p′-DDT and MXC regulated gonadotropin genes expression and hormones synthesis through ERK pathway in gonadotrope cells.