Choline-related supplements improve abnormal plasma methionine-homocysteine metabolites and glutathione status in children with cystic fibrosis
Choline-related supplements improve abnormal plasma methionine-homocysteine metabolites and glutathione status in children with cystic fibrosis
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DOI：
10.1093/ajcn/85.3.702
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发表时间：
2007-03-01
影响因子：
7.1
通讯作者：
James, S. Jill
中科院分区：
文献类型：
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作者：
Innis, Sheila M.;Davidson, A. George F.;James, S. Jill
背景：肝甘油三酯积累和氧化应激在囊性纤维化（CF）中很常见，胆碱缺乏也会发生。先前，我们发现cf患儿血浆同型半胱氨酸升高、s -腺苷蛋氨酸与s -腺苷同型半胱氨酸（SAM:SAH）和磷脂酰胆碱与磷脂酰乙醇胺比值降低与磷脂吸收不良之间存在关联。目的：目的是探讨cf患儿蛋氨酸-同型半胱氨酸代谢和胆碱代谢改变之间的可能关系。CF患儿被无偏倚地分配到补充2 g卵磷脂/d （n = 13）、2 g胆碱/d （n = 12）或3 g甜菜碱/d (n = 10)，持续14天。蛋氨酸、腺苷、半胱氨酸、半胱氨酸-甘氨酸、谷胱甘肽、谷胱甘肽二硫（GSSG）和脂肪酸的血浆浓度；山姆:SAH;测定各组CF患儿补充前后红细胞磷脂含量。对无CF的健康儿童（n = 15）的血浆进行分析以获得参考数据。结果：CF患儿血浆同型半胱氨酸、SAH和腺苷较高，而蛋氨酸、SAM:SAH和谷胱甘肽：GSSG较低。补充卵磷脂、胆碱或甜菜碱可显著增加血浆蛋氨酸、SAM、SAM:SAH和谷胱甘肽：GSSG，并降低SAH （n = 35）。补充胆碱或甜菜碱与血浆SAH的显著降低和SAM:SAH、蛋氨酸和谷胱甘肽：GSSG的增加相关。补充卵磷脂或胆碱也增加血浆蛋氨酸和SAM。结论：我们发现饮食中补充胆碱相关化合物可以改善CF患儿低SAM:SAH和谷胱甘肽氧化还原平衡。
Background: Liver triacylglycerol accumulation and oxidative stress are common in cystic fibrosis (CF) and also occur in choline deficiency. Previously, we showed an association between elevated plasma homocysteine, reduced ratios of S-adenosylmethionine to S-adenosylhomocysteine (SAM:SAH) and of phosphatidylcholine to phosphatidylethanolamine, and phospholipid malabsorption in children with CF.Objective: The objective was to address a possible relation between altered methionine-homocysteine metabolism and choline metabolism in children with CF.Design: Children with CF were assigned without bias to supplementation with 2 g lecithin/d (n = 13), 2 g choline/d (n = 12), or 3 g betaine/d (n = 10) for 14 d. Plasma concentrations of methionine, adenosine, cysteine, cysteinyl-glycine, glutathione, glutathione disulfide (GSSG), and fatty acids; SAM:SAH; and red blood cell phospholipids were measured within each group of children with CF before and after supplementation. Plasma from healthy children without CF (n = 15) was analyzed to obtain reference data.Results: Children with CF had higher plasma homocysteine, SAH, and adenosine and lower methionine, SAM:SAH, and glutathione: GSSG than did children without CF. Supplementation with lecithin, choline, or betaine resulted in a significant increase in plasma methionine, SAM, SAM:SAH, and glutathione:GSSG and a decrease in SAH (n = 35). Supplementation with choline or betaine was associated with a significant decrease in plasma SAH and an increase in SAM:SAH, methionine, and glutathione:GSSG. Supplementation with lecithin or choline also increased plasma methionine and SAM.Conclusion: We showed that dietary supplementation with choline-related compounds improves the low SAM:SAH and glutathione redox balance in children with CF.