Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis
Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis
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DOI：
10.3390/diagnostics10050333
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发表时间：
2020-05-01
期刊：
影响因子：
3.6
通讯作者：
Lu, Po-Liang
中科院分区：
文献类型：
--
作者：
Chen, Chao-Ju;Hsieh, Li-Ling;Lu, Po-Liang
2019冠状病毒病（COVID-19）是当前一种无法控制的传染病，在全球范围内造成了重大挑战。全世界都需要一种可靠的COVID-19快速诊断检测方法。实时逆转录酶聚合酶链是在新型病毒大流行中建立最快的方法之一，被认为是检测严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）的金标准。在本报告中，我们阐述了我们应用台湾疾病预防控制中心的方案并在紧急情况下实现分析优化的经验，以满足紧急的医疗和公共卫生需求。
Coronavirus disease 2019 (COVID-19), the current uncontrolled outbreak of infectious disease, has caused significant challenges throughout the world. A reliable rapid diagnostic test for COVID-19 is demanded worldwide. The real-time reverse transcriptase polymerase chain was one of the most quickly established methods in the novel viral pandemic and was considered as the gold standard for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this report, we illustrate our experience of applying a protocol from the Taiwan CDC and achieving assay optimization in the immediate circumstances to meet the urgent medical and public health needs.