Investigation of a short interspersed nuclear element polymorphic site in the porcine vertnin gene: allele frequencies and association study with meat quality, carcass and production traits in Italian Large White pigs
Investigation of a short interspersed nuclear element polymorphic site in the porcine vertnin gene: allele frequencies and association study with meat quality, carcass and production traits in Italian Large White pigs
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DOI：
10.4081/ijas.2014.3090
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发表时间：
2014-01-01
影响因子：
2.5
通讯作者：
Russo, Vincenzo
中科院分区：
文献类型：
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作者：
Fontanesi, Luca;Scotti, Emilio;Russo, Vincenzo
猪7号染色体上猪Vertnin(VRTN)基因291bp短的散布核素(Sine)插入影响了脊椎数和几个生产性状：与野生型等位基因(WT，未插入)相比，等位基因Q(插入)增加了脊椎数。在本研究中，我们对8个猪品种(意大利大白猪、意大利杜洛克猪、意大利长白猪、辛塔·塞内斯猪、Mora Romagnola猪、Casertana猪、Apulo Calabrese猪和尼罗·西西里亚诺猪)和意大利野猪进行了基因分型，以评估这两个等位基因的频率分布。等位基因Q在意大利长白猪和意大利杜洛克猪中的频率最高(分别为0.738和0.545)，而在所有其他品种中频率较低，在野猪中不存在。联合研究是在两个意大利大白样本中进行的。这两组动物是由性能测试猪组成的，计算了几个性状(平均日增重、背脂厚度、料重比、瘦肉率和火腿重)的估计育种值(EBV)和随机残差(RR)：i)在没有任何标准的情况下选择270头猪(随机组)，也测量了几个肉质性状；ii)背脂厚度的EBV极端和差异较大的560头母猪。这些动物的椎体数量是不可用的。关联分析的结果表明，等位基因Q与较低的火腿重量相关，间接证实了其他研究报告的脊椎数与该性状之间的负相关。没有其他性状与分析的VRTN多态相关。
A 291 bp short interspersed nuclear element (SINE) insertion in the porcine vertnin (VRTN) gene on porcine chromosome 7 was shown to affect vertebral number and several production traits: allele Q (with the insertion) increases vertebral number compared to the wild type allele (WT, without insertion). In this study we genotyped this polymorphism in eight pig breeds (Italian Large White, Italian Duroc, Italian Landrace, Cinta Senese, Mora Romagnola, Casertana, Apulo Calabrese, and Nero Siciliano) and in Italian wild boars to evaluate allele frequency distribution of the two alleles. Allele Q was the most frequent in Italian Landrace and Italian Duroc (0.738 and 0.545, respectively) whereas it was the less frequent in all other breeds and was absent in wild boars. Association study was carried out in two Italian Large White samples. These two groups of animals were constituted by performance tested pigs for which estimated breeding values (EBV) and random residuals (RR) for several traits (average daily gain, back fat thickness, feed: gain ratio, lean cuts and ham weight) were calculated: i) 270 pigs chosen without any criteria (random group), that were also measured for several meat quality traits; ii) 560 gilts with extreme and divergent EBV for back fat thickness. For these animals vertebral number was not available. Results of the association analyses indicated that allele Q was associated with a lower ham weight, confirming indirectly, the negative correlation reported by other studies between vertebral number and this trait. No other trait was associated with the analysed VRTN polymorphism.