Effects of a sub-minimum inhibitory concentration of chlorhexidine gluconate on the development of in vitro multi-species biofilms.
Effects of a sub-minimum inhibitory concentration of chlorhexidine gluconate on the development of in vitro multi-species biofilms.
复制标题
次最低抑制浓度的葡萄糖酸氯己定对体外多物种生物膜形成的影响。
DOI：
10.1080/08927014.2020.1739271
复制
发表时间：
2020
期刊：
影响因子：
2.7
通讯作者：
Noiri Y.
中科院分区：
文献类型：
--
作者：
Suzuki Y;Ohsumi T;Isono T;Nagata R;Hasegawa T;Takenaka S;Terao Y;Noiri Y.
在口腔环境中施用抗微生物剂后，细菌暴露于亚最小抑菌浓度（sub-MIC），这可以诱导体外单种生物膜。本研究探讨葡萄糖酸氯己定（CHG）在亚MIC浓度下对变形链球菌、口腔链球菌和内氏放线菌体外多菌种生物膜的影响。发现亚MIC的CHG可诱导体外生物膜生长，尽管细菌生长与对照组无显着差异。与S. CHG在亚MIC下的多菌种生物膜形成显著高于对照，但在单菌种生物膜中没有发现这一点。CHG在亚MIC时的胞外多糖生物量显著高于对照。这表明亚MIC浓度的CHG可能通过影响S相关基因的转录而促进多物种生物膜的形成。生物膜形成。
Following antimicrobial administrations in oral environments, bacteria become exposed to a sub-minimum inhibitory concentration (sub-MIC), which can inducein vitrosingle-species biofilms. This study explored the effects of chlorhexidine gluconate (CHG) at a sub-MIC onin vitromulti-species biofilms comprisingStreptococcus mutans,Streptococcus oralisandActinomyces naeslundii. CHG at a sub-MIC was found to inducein vitrobiofilm growth, although the bacterial growth was not significantly different from that in the control. The gene transcription related toS. mutansmulti-species biofilm formation with CHG at a sub-MIC was significantly higher than that of the control, but this was not found inS. mutanssingle-species biofilms. The bio-volume of extracellular polysaccharides with CHG at a sub-MIC was significantly higher than that of the control. This suggests that CHG at a sub-MIC may promote the development of multi-species biofilms by affecting the gene transcription related toS. mutansbiofilm formation.