PREDICTION OF REPETITIVE FIRING BEHAVIOUR FROM VOLTAGE CLAMP DATA ON AN ISOLATED NEURONE SOMA
PREDICTION OF REPETITIVE FIRING BEHAVIOUR FROM VOLTAGE CLAMP DATA ON AN ISOLATED NEURONE SOMA
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DOI：
10.1113/jphysiol.1971.sp009366
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发表时间：
1971-01-01
影响因子：
5.5
通讯作者：
STEVENS, CF
中科院分区：
文献类型：
--
作者：
CONNOR, JA;STEVENS, CF
1. 用电压箝位法测定了离体神经细胞体的膜参数。数据表示为膜离子特异性电导，泄漏电导和电容。2. 存在三种离子电流：向内，II；以及两个操作上不同的外向电流，IK和IA。两种向外的电流显然都是由钾离子携带的。3. 求解了两个连续动作电位在恒定刺激电流下放电的霍奇金-赫胥黎方程。将数字计算机解与所研究细胞记录的动作电位数据进行比较。4. 计算和实验测量的发射频率和刺激电流强度之间的关系进行了比较，并且在总频率范围的同一部分是线性的。5. 细胞行为在峰间间隔的后期由电导gA主导，而gNa和gK在很大程度上决定了动作电位的特征和峰间间隔的初始部分。6. 膜的预超极化可激活gA，膜对去极化电流的响应与未施加预超极化时的响应明显不同。
1. Membrane parameters of an isolated neural cell body have been determined by voltage clamp analysis. Data are expressed as membrane ion-specific conductances, leak conductance, and capacitance. 2. Three ionic currents are present: Inward, II; and two operationally distinct outward currents, IK and IA. Both outward currents are apparently carried by potassium ions. 3. Hodgkin—Huxley-like equations were solved for the discharge of two sequential action potentials in response to a constant stimulus current. The digital computer solutions are compared with action potential data recorded from the investigated cell. 4. The computed and experimentally measured relationships between firing frequency and stimulus current intensity are compared and are linear over the same portion of the total frequency range. 5. Cell behaviour in the latter part of the interspike interval is dominated by the conductance gA while gNa and gK largely determine the character of the action potential and the initial portion of the interspike interval. 6. Prehyperpolarization of the membrane activates gA and the membrane response to depolarizing current differs markedly from the response elicited when no prehyperpolarization is imposed.