THREE-DIMENSIONAL RECONSTRUCTION AND VISUALIZATION OF THE MEDIAN NERVE FROM SERIAL TISSUE SECTIONS
THREE-DIMENSIONAL RECONSTRUCTION AND VISUALIZATION OF THE MEDIAN NERVE FROM SERIAL TISSUE SECTIONS
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DOI：
10.1002/micr.20646
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发表时间：
2009-01-01
期刊：
影响因子：
2.1
通讯作者：
Hu, Ping
中科院分区：
文献类型：
--
作者：
Sun, Kuo;Zhang, Jian;Hu, Ping
本研究的目的是通过乙酰胆碱酯酶(AChE)组织化学染色，在计算机技术的辅助下，明确地实现人正中神经感觉束和运动束的三维可视化。新鲜的人正中神经取自一具成年男性身体。正中神经固定在一个特殊的固定器上。然后，整个保持器被嵌入并迅速冷冻在液氮中。将处理后的正中神经在滑动冰冻切割机上沿其长轴每隔100微米、厚度20微米进行冠状切断术。切片总数为4650片。所有切片均行AChE组织化学染色。染色的切片被扫描并保存为联合图像专家组文件。这些带有正负染色切片的图像被采集到Intel Dual Pentium计算机上。用Adobe Photoshop CS2软件比较染色前后图像的参考点。获得正中神经的二维神经内微结构数据库。开发了神经三维可视化系统软件。利用神经三维可视化系统，生成正中神经神经内微结构的三维可视化结果。本研究结果可为神经修复，特别是束神经修复提供更准确、更详细的解剖学信息。神经三维可视化技术可能对未来周围神经的地形学研究具有潜在的应用价值。(C)2009年Wiley-Liss，Inc.显微外科29：573-577,2009。
The purpose of this study was to definitively implement the three-dimensional visualization of sensory and motor fascicles in the human median nerve by means of acetylcholinesterase (AChe) histochemical staining and under the assistance of the computer technology. One fresh human median nerve was harvested from a male adult cadaver. The median nerve was fixed at a special holder. Then, the whole holder was embedded and rapidly frozen in the liquid nitrogen. The processed median nerve was then cut coronally every 100 mu m at a 20 mu m thickness along its long axis in a sliding freezing microtome. The total number of sections was 4,650 slices. All sections were stained with the AChe histochemical method. The stained sections were scanned and saved as Joint Photographic Experts Group files. These images with positively and negatively stained sections were acquired to an Intel dual Pentium computer. The Adobe Photoshop CS2 software was used to compare the reference points of images before and after staining. The two-dimensional intraneural microstructure database of median nerve was then acquired. A software of 3D nerve visualization system was developed. With the 3D nerve visualization system, the 3D visualization result of intraneural microstructure of median nerve was created. The findings may provide more accurate and detailed anatomic information for nerve repairs, specifically for the fascicular nerve repairs. The 3D nerve visualization technique may have potential for future studies of topography of peripheral nerve. (C) 2009 Wiley-Liss, Inc. Microsurgery 29:573-577, 2009.