Microglia promote glioma migration
Microglia promote glioma migration
复制标题
DOI：
10.1007/s00401-001-0472-x
复制
发表时间：
2002-04-01
影响因子：
12.7
通讯作者：
Paulus, W
中科院分区：
文献类型：
--
作者：
Bettinger, I;Thanos, S;Paulus, W
弥漫性星形细胞胶质瘤广泛浸润脑组织，并含有大量的小胶质细胞，但这两个特征是否在病理学上相关尚不清楚。因此，我们研究了小鼠小胶质细胞的运动的GL 261小鼠胶质瘤细胞使用Boyden室测定的影响。在存在小胶质细胞的情况下，胶质瘤细胞迁移发生得更早，并且在48小时后，与没有小胶质细胞的孵育相比，其是三倍。这种作用是由小胶质细胞释放的物质介导的，因为小胶质细胞条件培养基观察到类似的作用，并且它是小胶质细胞特异性的，因为少突胶质细胞和内皮细胞仅微弱地刺激胶质细胞瘤细胞迁移。小胶质细胞激活物质（GM-CSF，LPS）导致运动进一步增加。这些数据支持这样的观点，即弥漫性神经胶质瘤中的小胶质细胞积聚不仅代表了对组织损伤的非特异性反应，而且反映了这些细胞参与支持和促进星形细胞瘤细胞的侵袭性表型。
Diffuse astrocytic gliomas extensively infiltrate brain tissue and contain numerous microglial cells, but it is unknown whether these two characteristic features are pathogenetically related. We therefore studied the effects of murine microglial cells on motility of GL261 mouse glioma cells using Boyden chamber assays. In the presence of microglia, glioma cell migration occurred earlier, and after 48 h it was threefold higher as compared to incubations without microglia. This effect was mediated by substances released from microglia, because similar effects were observed by microglia-conditioned medium, and it was specific to microglia, because oligodendroglia and endothelial cells only weakly stimulated glioma cell migration. Microglia activating substances (GM-CSF, LPS) led to a further increase of motility. These data support the notion that microglia accumulation in diffuse glial tumors does not merely represent a nonspecific reaction to tissue injury, but reflects participation of these cells in supporting and promoting the invasive phenotype of astrocytoma cells.