Chronic transplantation of olfactory ensheathing cells promotes partial recovery after complete spinal cord transection in the rat
Chronic transplantation of olfactory ensheathing cells promotes partial recovery after complete spinal cord transection in the rat
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DOI：
10.1002/glia.20457
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发表时间：
2007-02-01
期刊：
影响因子：
6.2
通讯作者：
Verdu, Enrique
中科院分区：
文献类型：
--
作者：
Lopez-Vales, Ruben;Fores, Joaquim;Verdu, Enrique
本研究的目的是确定嗅鞘细胞（OECs）是否能够促进轴突再生和功能恢复时，移植45天后完全横断的成年大鼠胸脊髓。嗅鞘细胞促进脊髓上的运动诱发电位和后肢运动的适度恢复评估路径的部分恢复。此外，OEC移植物从移植后第一个月开始减少腰椎反射性过度兴奋。组织学结果显示，嗅鞘细胞促进皮质脊髓和中缝脊髓轴突再生通过损伤部位，并进入脊髓尾段。有趣的是，中缝脊神经纤维而不是皮质脊髓纤维通过灰质长距离再生，并到达脊髓的下腰段（L5）。然而，延迟OEC移植未能减少创伤后星形胶质细胞增生。总之，本研究中发现的有益效果进一步支持使用嗅鞘细胞治疗慢性脊髓损伤。(c)2006 Wiley-Liss，Inc.
The goal of this study was to ascertain whether olfactory ensheathing cells (OECs) were able to promote axonal regeneration and functional recovery when transplanted 45 days after complete transection of the thoracic spinal cord in adult rats. OECs promoted partial restitution of supraspinal pathways evaluated by motor evoked potentials and modest recovery of hindlimb movements. In addition, OEC grafts reduced lumbar reflex hyperexcitability from the first month after transplantation. Histological results revealed that OECs facilitated corticospinal and raphespinal axons regrowth through the injury site and into the caudal spinal cord segments. Interestingly, raphespinal but not corticospinal fibers regenerated long distances through the gray matter and reached the lower lumbar segments (L5) of the spinal cord. However, delayed OEC grafts failed to reduce posttraumatic astrogliosis. In conclusion, the beneficial effects found in the present study further support the use of OECs for treating chronic spinal cord injuries. (c) 2006 Wiley-Liss, Inc.