Effect of myostatin on chemokine expression in regenerating skeletal muscle cells.
Effect of myostatin on chemokine expression in regenerating skeletal muscle cells.
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肌肉生长抑制素对再生骨骼肌细胞趋化因子表达的影响。
DOI：
10.1159/000351462
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Aso H.
中科院分区：
文献类型：
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作者：
Iwasaki S;Miyake M;Hayashi S;Watanabe H;Nagasawa Y;Terada S;Watanabe K;Ohwada S;Kitazawa H;Rose MT;Aso H.
转化生长因子-β（TGF-β）参与调控趋化因子的表达，所述趋化因子控制肌发生中的多个步骤。然而，TGF-β超家族成员肌生长抑制素是否影响骨骼肌中趋化因子的表达仍有待确定。我们研究了肌肉生长抑制素对正常肌肉（NM）和双肌（DM）牛完整和再生胸最长肌中4种趋化因子（CXCL 1、CXCL 2、CXCL 6、CCL 2）mRNA和蛋白表达的影响。这些趋化因子在再生肌肉中表达，并且它们的表达在DM中总是低于NM牛。免疫组化结果显示，在NM牛和DM牛的成肌细胞和肌管的再生区检测到CXCL 1和CXCL 6。从再生肌肉分离的成肌细胞培养物中，在增殖阶段（P期），DM成肌细胞中CXCL 1、CXCL 2和CCL 2 mRNA的表达显著低于NM成肌细胞。NM成肌细胞中CXCL 1、CXCL 2和CCL 2 mRNA的表达以及DM成肌细胞中CXCL 1、CXCL 2和CXCL 6 mRNA的表达在从P期转换到融合期（F期）时降低。此外，在F期，DM中CXCL 1、CXCL 2和CXCL 6 mRNA的表达显著低于NM成肌细胞。在F期加入100 ng/ml肌肉生长抑制素减弱了CXCL 1和CXCL 2 mRNA的表达，并增强了CCL 2的表达。这些结果首次表明，肌肉生长抑制素调节骨骼肌细胞中趋化因子的差异表达。
Transforming growth factor-β (TGF-β) is implicated in the regulatory expression of chemokines that control multiple steps in myogenesis. However, it remains to be established whether myostatin, a member of the TGF-β superfamily, affects chemokine expression in skeletal muscle. We investigated the effects of myostatin on the expression of mRNAs and proteins for 4 chemokines (CXCL1, CXCL2, CXCL6, CCL2) in intact and regenerating musculus longissimus thoracis from normal-muscled (NM) and double-muscled (DM) cattle. These chemokines were expressed in regenerating muscle, and their expression was always lower in DM than in NM cattle. Immunohistochemistry revealed that CXCL1 and CXCL6 were detected in the regenerating areas of myoblasts and myotubes in both NM and DM cattle. In cultures of myoblasts isolated from the regenerating muscles, significantly less CXCL1, CXCL2 and CCL2 mRNA was expressed in DM myoblasts than in NM myoblasts during the proliferating stage (P-stage). The expression of CXCL1, CXCL2 and CCL2 mRNAs in NM myoblasts and CXCL1, CXCL2 and CXCL6 mRNAs in DM myoblasts decreased upon switching from P-stage to fusion stage (F-stage). Also, the expression of CXCL1, CXCL2 and CXCL6 mRNAs was significantly lower in DM than in NM myoblasts during the F-stage. The addition of 100 ng/ml myostatin during the F-stage attenuated the expression of CXCL1 and CXCL2 mRNAs and augmented that of CCL2. These results show for the first time that myostatin regulates the differential expression of chemokines in skeletal muscle cells.