Inflammatory profile in a canine model of hypothermic circulatory arrest.
Inflammatory profile in a canine model of hypothermic circulatory arrest.
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DOI：
10.1016/j.jss.2021.02.014
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Wilson MA
中科院分区：
文献类型：
--
作者：
Giuliano K;Torres-Odio S;Etchill E;Carr P;Conover Talbot C Jr;Blue ME;Johnston MV;Baumgartner WA;Lawton JS;Wilson MA
低温循环停止（HCA）是一种用于主动脉复杂修复的技术，但它可能与神经系统疾病有关。为了更好地了解缺血性脑损伤背后的分子变化，我们评估了接受2小时HCA的犬脑组织和脑脊液（CSF）中的基因表达和细胞因子/趋化因子多肽浓度。成年公犬外周插管进行体外循环，冷却至18°C，停搏2小时。动物在hca后2小时、8小时或24小时被安乐死（每组n=8），并使用基因表达微阵列分析、细胞因子测定和组织病理学将它们的大脑与8只正常犬的大脑进行比较。HCA后2 ~ 8小时，促炎细胞因子mrna显著增加，与神经炎症或缺血性损伤相关的信号通路中基因表达丰富。正常犬脑中促炎细胞因子多肽IL-6、IL-8、IL-1β和CCL2的表达水平极低，抗炎细胞因子IL-10和TGF-β1的表达水平中等。HCA后脑组织和脑脊液中促炎细胞因子浓度明显升高。IL-6和IL-8在8小时达到峰值，24小时下降，而IL-1β和CCL2保持升高。抗炎IL-10和TGF-β1的浓度在HCA后维持不变，TGF-β1在24小时显著升高。这些细胞因子代表了缺血性神经损伤的潜在诊断标志物，可用于评估HCA患者的神经损伤。这种促炎症、缺血性损伤的细胞机制是未来神经保护的潜在目标。
Hypothermic circulatory arrest (HCA) is a technique used for complex repair of the aorta, but it can be associated with neurologic morbidity. To better understand the molecular changes that underlie ischemic brain injury, we assessed gene expression and cytokine/chemokine polypeptide concentration in brain tissue and cerebrospinal fluid (CSF) of canines that underwent two hours of HCA. Adult male canines were cannulated peripherally for cardiopulmonary bypass, cooled to 18°C, and arrested for two hours. Animals were euthanized two, eight, or 24 hours post-HCA (n=8 per group), and their brains were compared to brains from eight normal canines, using gene expression microarray analysis, cytokine assay, and histopathology. Two to eight hours after HCA, pro-inflammatory cytokine mRNAs increased markedly, and gene expression was enriched within signaling pathways related to neuroinflammation or ischemic injury. Concentrations of pro-inflammatory cytokine polypeptides IL-6, IL-8, IL-1β, and CCL2 were very low in normal canine brain, whereas anti-inflammatory IL-10 and TGF-β1 were expressed at moderate levels. Pro-inflammatory cytokine concentrations rose robustly in cerebral tissue and CSF after HCA. IL-6 and IL-8 peaked at eight hours and declined at 24 hours, while IL-1β and CCL2 remained elevated. Concentrations of anti-inflammatory IL-10 and TGF-β1 were maintained after HCA, with a significant increase in TGF-β1 at 24 hours. These cytokines represent potential diagnostic markers for ischemic neurologic injury that could be used to assess neurologic injury in patients undergoing HCA. The cellular mechanisms underlying this pro-inflammatory, ischemic-induced injury represent potential targets for neuroprotection in the future.
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DOI：
10.1097/nen.0000000000000134
发表时间：
2014-12
影响因子：
3.2
作者：
Blue ME;Wilson MA;Beaty CA;George TJ;Arnaoutakis GJ;Haggerty KA;Jones M;Brawn J;Manmohan S;Lange MS;Johnston MV;Baumgartner WA;Troncoso JC
通讯作者：
Troncoso JC
影响因子：
4.6
作者：
Allen, Jeremiah G.;Weiss, Eric S.;Wilson, Mary Ann;Arnaoutakis, George J.;Blue, Mary E.;Talbot, C. Conover, Jr.;Jie, Chunfa;Lange, Mary S.;Troncoso, Juan C.;Johnston, Michael V.;Baumgartner, William A.
通讯作者：
Baumgartner, William A.
DOI：
10.1016/j.athoracsur.2016.02.077
发表时间：
2016-09
期刊：
The Annals of thoracic surgery
影响因子：
--
作者：
Grimm JC;Magruder JT;Wilson MA;Blue ME;Crawford TC;Troncoso JC;Zhang F;Kannan S;Sciortino CM;Johnston MV;Kannan RM;Baumgartner WA
通讯作者：
Baumgartner WA
影响因子：
5.2
作者：
Harari OA;Liao JK
通讯作者：
Liao JK
DOI：
10.1186/s12950-014-0026-3
发表时间：
2014
期刊：
Journal of inflammation (London, England)
影响因子：
--
作者：
Engels M;Bilgic E;Pinto A;Vasquez E;Wollschläger L;Steinbrenner H;Kellermann K;Akhyari P;Lichtenberg A;Boeken U
通讯作者：
Boeken U