Delayed preconditioning effect of isoflurane on spinal cord ischemia in rats
Delayed preconditioning effect of isoflurane on spinal cord ischemia in rats
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DOI：
10.1016/j.neulet.2008.05.097
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发表时间：
2008-08
影响因子：
2.5
通讯作者：
Hong Kim;J. Yi;Yun-Hee Sung;Chang-Ju Kim;Chong-Sung Kim;Jong-Man Kang
中科院分区：
文献类型：
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作者：
Hong Kim;J. Yi;Yun-Hee Sung;Chang-Ju Kim;Chong-Sung Kim;Jong-Man Kang
截瘫是主动脉瘤手术后最常见的并发症之一。本研究旨在探讨异氟醚诱导的大鼠脊髓延迟预适应是否由核因子κB（NF-κB）介导。将动物分为4组：对照组、吡咯烷二硫代氨基甲酸酯（PDTC，NF-κB抑制剂）处理组、异氟烷处理组和PDTC/异氟烷处理组。PDTC治疗组于术前1h、再灌注后24 h和48 h腹腔注射2%PDTC 100 mg/kg。异氟醚治疗组于脊髓缺血前24 h吸入2.8%异氟醚30 min。与对照组相比，NF-κB抑制剂预处理显著降低NF-κB表达和完整运动神经元的数量。与异氟烷处理组相比，异氟烷预处理增加了正常运动神经元的数量，而PDTC和异氟烷预处理显著减少了它们。异氟醚诱导的脊髓缺血延迟预处理改善了组织病理学结果。异氟醚预处理对脊髓缺血的神经保护作用与NF-κB表达有关。
Paraplegia is one of the most common complications following aortic aneurysmal surgery. This study was designed to determine if isoflurane-induced delayed preconditioning is mediated by nuclear factor κB (NF-κB) in the rat spinal cord. The animals were divided into four groups: the control group, the pyrrolidinedithio carbamate (PDTC, an NF-κB inhibitor)-treated group, the isoflurane-treated group, and the PDTC/isoflurane-treated group. In the PDTC-treated groups, 2% 100mg/kg PDTC was administered intraperitoneally at 1h before operation and at 24h and 48h after reperfusion. The rats in the isoflurane-treated groups received 30min inhalation of 2.8% isoflurane at 24h before spinal cord ischemia. Pretreatment with NF-κB inhibitor significantly reduced NF-κB expression and the number of intact motor neurons when compared to the control group. Preconditioning with isoflurane increased the number of normal motor neurons, whereas pretreatment with both PDTC and isoflurane significantly decreased them, compared to the isoflurane-treated group. Isoflurane-induced delayed preconditioning on spinal cord ischemia improved histopathological outcomes. This neuroprotective effect of isoflurane preconditioning on spinal cord ischemia is associated with NF-κB expression.