Spermidine and Spermine Are Enriched in Whole Blood of Nona/Centenarians
Spermidine and Spermine Are Enriched in Whole Blood of Nona/Centenarians
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DOI：
10.1089/rej.2012.1349
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发表时间：
2012-12-01
影响因子：
2.6
通讯作者：
Napolioni, Valerio
中科院分区：
文献类型：
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作者：
Pucciarelli, Stefania;Moreschini, Benedetta;Napolioni, Valerio
多胺(腐胺、亚精胺和精胺)是由鸟氨酸通过脱羧基过程衍生出来的一类分子。它们对细胞的生长和增殖、稳定DNA负电荷、RNA转录、翻译和细胞凋亡都是必不可少的。最近，在酵母、苍蝇、蠕虫和人类培养的免疫细胞中，已经证明外源性给予亚精胺可以延长寿命。在这里，我们使用横断面观察性研究，测定了78名性别匹配的无关个体的全血多胺水平，将其分为三个年龄组：组1(31-56岁，n=26，平均年龄44.6±6.07岁)，组2(60-80岁，n=26，平均年龄68.7±6.07岁)，组3(90-106岁，n=26，平均年龄96.5±4.59岁)。组2和组3的总多胺含量显著低于组1(p=3.6×10(-12))。有趣的是，这种减少主要是由于腐胺含量较低所致。组2的精胺和精胺水平最低。另一方面，与其他组相比，NONA/百岁老人(第3组)的精胺相对含量的中位数明显更高(13.2%比14.1%比30.6%，p=6.0×10(-4))。我们第一次报告了健康的NONA/百岁老人全血中多胺的情况，我们的结果证实并扩展了之前关于多胺在决定人类寿命方面的作用的发现。然而，尽管我们发现多胺水平和年龄组之间存在重要的相关性，但仍有必要进行进一步的研究，以充分了解多胺在决定寿命方面的作用。此外，纵向和营养研究可能会提出潜在的治疗方法，以维持健康的衰老和延长人类的寿命。
Polyamines (putrescine, spermidine, and spermine) are a family of molecules that derive from ornithine through a decarboxylation process. They are essential for cell growth and proliferation, stabilization of negative charges of DNA, RNA transcription, translation, and apoptosis. Recently, it has been demonstrated that exogenously administered spermidine promotes longevity in yeasts, flies, worms, and human cultured immune cells. Here, using a cross-sectional observational study, we determined whole-blood polyamines levels from 78 sex-matched unrelated individuals divided into three age groups: Group 1 (31-56 years, n = 26, mean age 44.6 +/- 6.07), group 2 (60-80 years, n = 26, mean age 68.7 +/- 6.07), and group 3 (90-106 years, n = 26, mean age 96.5 +/- 4.59). The total content of polyamines is significantly lower in groups 2 and 3 compared to group 1 (p = 3.6 x 10(-12)). Interestingly, this reduction is mainly attributable to the lower putrescine content. Group 2 displays the lowest levels of spermidine and spermine. On the other hand, nona/centenarians (group 3) display a significantly higher median relative percentage content of spermine with respect to total polyamines, compared to the other groups (13.2% vs. 14.1% vs. 30.6%, p = 6.0 x 10(-4)). For the first time, we report profiles of polyamines from the whole blood of healthy nona/centenarians, and our results confirm and extend previous findings on the role of polyamines in determining human longevity. However, although we found an important correlation between polyamines levels and age groups, further studies are warranted to fully understand the role of polyamines in determining life span. Also, longitudinal and nutritional studies might suggest potential therapeutic approaches to sustain healthy aging and to increase human life span.