Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton.
Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton.
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DOI：
10.1083/jcb.201512029
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发表时间：
2016-04-11
期刊：
影响因子：
--
通讯作者：
Gladfelter AS
中科院分区：
文献类型：
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作者：
Bridges AA;Jentzsch MS;Oakes PW;Occhipinti P;Gladfelter AS
真菌和人类septins可以区分细胞中不同程度的微米级曲率，这表明septins细胞骨架的这种特性为细胞提供了一种感知其局部形状的机制。细胞会根据不同的环境和发育条件改变形状，形成具有微米级特征的拓扑结构。虽然单个蛋白质可以感知纳米级的膜曲率，但尚不清楚细胞是否也可以使用纳米级的组件来感知微米级的轮廓，例如神经元分支的细胞动力学沟和基底。隔膜蛋白是作为信号平台的促细胞生成蛋白，并且经常与存在微米级曲率的质膜区域相关，包括细胞动力学沟和细胞突起的基部。我们在这里报告说，真菌和人类septins能够区分不同程度的微米级曲率的细胞。通过在不同曲率的珠子上制备支持的脂质双层，我们重建并测量了固有的septin曲率偏好。我们的结论是，微米级的曲率识别是一个基本属性的隔蛋白细胞骨架，提供了一个机制，知道它的局部形状的细胞。
Fungal and human septins can distinguish between different degrees of micron-scale curvature in cells, suggesting that this property of the septin cytoskeleton provides a cell with a mechanism to sense its local shape. Cells change shape in response to diverse environmental and developmental conditions, creating topologies with micron-scale features. Although individual proteins can sense nanometer-scale membrane curvature, it is unclear if a cell could also use nanometer-scale components to sense micron-scale contours, such as the cytokinetic furrow and base of neuronal branches. Septins are filament-forming proteins that serve as signaling platforms and are frequently associated with areas of the plasma membrane where there is micron-scale curvature, including the cytokinetic furrow and the base of cell protrusions. We report here that fungal and human septins are able to distinguish between different degrees of micron-scale curvature in cells. By preparing supported lipid bilayers on beads of different curvature, we reconstitute and measure the intrinsic septin curvature preference. We conclude that micron-scale curvature recognition is a fundamental property of the septin cytoskeleton that provides the cell with a mechanism to know its local shape.