Digital quantification using amplified single-molecule detection
Digital quantification using amplified single-molecule detection
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DOI：
10.1038/nmeth916
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发表时间：
2006-09-01
期刊：
影响因子：
48
通讯作者：
Nilsson, Mats
中科院分区：
文献类型：
--
作者：
Jarvius, Jonas;Melin, Jonas;Nilsson, Mats
我们描述了一种生物分子计数的方案，通过将滚圈扩增介导的纳米级特定分子识别事件转换为适于离散光学检测的微米级荧光DNA分子。我们放大的单分子检测(SMD)方法保留了分子种群的离散性质，允许在七个数量级的动态范围内对分子进行多重检测和高精度量化。我们将该方法应用于霍乱弧菌的灵敏检测和定量。
We describe a scheme for biomolecule enumeration by converting nanometer-scale specific molecular recognition events mediated by rolling-circle amplification to fluorescent micrometer-sized DNA molecules amenable to discrete optical detection. Our amplified single-molecule detection (SMD) approach preserves the discrete nature of the molecular population, allowing multiplex detection and highly precise quantification of molecules over a dynamic range of seven orders of magnitude. We apply the method for sensitive detection and quantification of the bacterial pathogen Vibrio cholerae.