Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells
Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells
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DOI：
10.1016/j.chembiol.2006.06.013
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发表时间：
2006-07-01
影响因子：
--
通讯作者：
Grinstaff, Mark W.
中科院分区：
文献类型：
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作者：
Kenan, Daniel J.;Walsh, Elisabeth B.;Grinstaff, Mark W.
设计、合成了含有聚苯乙烯粘附肽结构域和细胞排斥性聚乙二醇结构域的两亲性大分子，并作为疏细胞表面涂层进行了评估。这种疏细胞或细胞排斥的涂层对于各种医学和生物技术应用都很感兴趣。使用 M13 噬菌体展示文库鉴定聚苯乙烯结合肽结构域的组成。使用 ELISA 和原子力光谱法评估两亲肽结构域与聚苯乙烯的结合亲和力。当涂在聚苯乙烯上时，两亲物降低了两种不同哺乳动物细胞系和致病性金黄色葡萄球菌菌株的细胞粘附。
Amphiphilic macromolecules containing a polystyrene-adherent peptide domain and a cell-repellent poly(ethylene glycol) domain were designed, synthesized, and evaluated as a cytophobic surface coating. Such cytophobic, or cell-repellent, coatings are of interest for varied medical and biotech nological applications. The composition of the polystyrene binding peptide domain was identified using an M13 phage display library. ELISA and atomic force spectroscopy were used to evaluate the binding affinity of the amphiphile peptide domain to polystyrene. When coated onto polystyrene, the amphiphile reduced cell adhesion of two distinct mammalian cell lines and pathogenic Staphylococcus aureus strains.