Role of proteoglycans in renal development.
Role of proteoglycans in renal development.
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蛋白多糖在肾脏发育中的作用。
DOI：
10.1016/0012-1606(88)90289-8
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发表时间：
1988
影响因子：
2.7
通讯作者：
Kanwar,YS
中科院分区：
文献类型：
--
作者：
Lelongt,B;Makino,H;Dalecki,TM;Kanwar,YS
研究了蛋白多糖（PGs）在形态发生中的作用。从13日龄的小鼠胚胎中获得胎肾，并在培养物中维持7天。在培养基中加入对硝基苯基-β-d-吡喃木糖苷可干扰PGs的生物合成。处理肾脏进行形态学和生化研究。形态学研究包括用抗基底膜抗体和钌红对组织进行染色。[35 S]硫酸盐用作生物合成和放射自显影研究的前体产物。木糖苷处理的肾脏间质疏松，输尿管芽分支抑制，肾单位发育减少，抗蛋白多糖抗体免疫荧光和钌红染色减少，[35 S]硫酸盐掺入组织不良的细胞外基质减少。生化研究包括通过Sepharose CL-4 B、-6B和DEAE-Sephacel色谱和醋酸纤维素电泳表征PG/糖胺聚糖（GAG）。在木糖苷的影响下，组织中的总放射性降低2至4倍，培养基组分中的总放射性增加18至42倍。PG的大分子形式的尺寸减小，即，分子量从2.5 × 106 ~ 2.5 × 104。合成的PG/GAG主要由硫酸乙酰肝素和少量硫酸软骨素组成。与对照相比，它们在较低的盐浓度下洗脱。媒体PG大小的类似减少，即，分子量在1.8 × 105 ~ 2.8 × 104之间。用[3 H]木糖苷进行的其他研究表明，木糖苷残基上起始的链在大小和组成上与GAG链相似。这些发现表明，PGs/GAGs生物合成的扰动导致肾器官发生异常。
The role of proteoglycans (PGs) in morphogenesis was investigated. Fetal kidneys were obtained from 13-day-old mouse embryos and maintained for 7 days in culture. The biosynthesis of PGs was perturbed by addition ofp-nitrophenyl-β-d-xylopyranoside in the culture medium. The kidneys were processed for morphological and biochemical studies. The morphological studies included staining of tissues with anti-basement membrane antibodies and ruthenium red. [35S]sulfate was used as the precursor product for biosynthetic and autoradiographic studies. The kidneys treated with xyloside had loose mesenchyme, inhibition of ureteric bud branching, diminution in the population of developing nephron elements, decreased immunofluorescence with anti-proteoglycan antibodies and staining with ruthenium red, and a reduced [35S]sulfate incorporation into poorly organized extracellular matrices. The biochemical studies included characterization of PGs/glycosaminoglycans (GAGs) by Sepharose CL-4B, -6B, and DEAE-Sephacel chromatographies and cellulose acetate electrophoresis. Under the influence of xyloside, the total radioactivities decreased 2 to 4-fold in tissues and increased 18 to 42-fold in media fractions. A reduction in the size of macromolecular form of PGs, i.e., from MW ∼2.5 × 106to ∼2.5 × 104, was noted. The PGs/GAGs synthesized were mainly made up of heparan sulfate and small amounts of chondroitin sulfate. They eluted at a lower salt concentration as compared to the controls. A similar diminution in the size of media PGs, i.e., from MW ∼1.8 × 105to ∼2.8 × 104, was observed. Additional studies with [3H]xyloside indicated that the chains initiated on xyloside residues were similar in size and composition to GAG-chains. These findings indicate that a perturbance in the biosynthesis of PGs/GAGs leads to abnormalities in renal organogenesis.
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7S 结构域构成 IV 型胶原蛋白的氨基末端：一项免疫化学研究。
DOI：
10.1016/s0174-173x(83)80008-9
发表时间：
1983
期刊：
Collagen and related research
影响因子：
--
作者：
Dixit,SN;Mainardi,CL;Beachey,EH;Kang,AH
通讯作者：
Kang,AH
肾小球滤过和蛋白尿的生物生理学。
DOI：
--
发表时间：
1984
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Kanwar,YS
通讯作者：
Kanwar,YS
从头合成的硫酸化糖胺聚糖在肾小球基底膜和系膜基质中的分布。
DOI：
--
发表时间：
1983
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Kanwar,YS;Rosenzweig,LJ;Jakubowski,ML
通讯作者：
Jakubowski,ML
体视学在分析细胞和器官的结构和功能方面的价值*
影响因子：
2
作者：
E. Weibel
通讯作者：
E. Weibel
胶原蛋白参与胚胎肺和唾液腺的分支形态发生。
影响因子：
2.7
作者：
B. Spooner;J. Faubion
通讯作者：
J. Faubion