Prediction of pocket-portable and implantable glucose enzyme electrode performance from combined species permeability and digital stimulation analysis.
Prediction of pocket-portable and implantable glucose enzyme electrode performance from combined species permeability and digital stimulation analysis.
复制标题
通过组合物种渗透性和数字刺激分析来预测袖珍便携式和植入式葡萄糖酶电极性能。
DOI：
10.1021/ac00081a026
复制
发表时间：
1994
影响因子：
7.4
通讯作者：
Updike,SJ
中科院分区：
文献类型：
--
作者：
Rhodes,RK;Shults,MC;Updike,SJ
酶电极传感器的开发可以通过使用计算机模拟建模技术来促进。在这项工作中，themodel帐户的接触溶液的影响，物种扩散和分配系数的变化，跨多个膜层界面，辅因子限制的影响，酶加载，酶的动力学和衰减，以及电极层的几何形状的其他变化。实验确定的单一物种的膜渗透性包括在数字模拟预测的多层葡萄糖氧化酶为基础的酶电极的性能特征。所获得的信息包括在不同底物和辅因子浓度组合下的传感器线性范围，渗透性参数和酶负载变化时的传感器时间响应和输出，传感器洗脱和干扰效应，以及传感器酶浓度衰减的预测和作为接触溶液条件和传感器渗透性的函数的所得估计传感器寿命。
Development of enzyme electrode sensors can be facilitated by the use of computer simulation modeling techniques. In this work, themodel accounts for contacting solution effects, changes in species diffusion and partition coefficients across multiple membrane layer interfaces, cofactor limitation effects, enzyme loading, enzyme kinetics and decay, and other variations in electrode layer geometry. Experimentally determined single species membrane permeabilities are included in the digital simulations to predict the performance characteristics of a multilayered glucose oxidasebased enzyme electrode. Infor-mation obtained includes range of sensor linearity under different substrateand cofactor concentration combinations, sensor time response and output as permeability parameters and enzyme loading are varied, sensor washout and interference effects, and prediction of decay of sensor enzyme concentration and resultant estimated sensor lifetime as a function of contacting solution conditions and sensor permeability.