Acute and late changes in N-acetyl-aspartate following diffuse axonal injury in rats: an MRI spectroscopy and microdialysis study.
Acute and late changes in N-acetyl-aspartate following diffuse axonal injury in rats: an MRI spectroscopy and microdialysis study.
复制标题
大鼠弥漫性轴突损伤后 N-乙酰基-天冬氨酸的急性和晚期变化：MRI 光谱和微透析研究。
DOI：
10.1080/01616412.2000.11740744
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发表时间：
2000
影响因子：
1.9
通讯作者：
Bullock,RM
中科院分区：
文献类型：
--
作者：
Alessandri,B;al-Samsam,R;Corwin,F;Fatouros,P;Young,HF;Bullock,RM
N-acetyl-aspartate (NAA) measured by proton nuclear magnetic resonance spectroscopy (1H-NMR) has been used as a marker of neuronal injury in many cerebral pathologies. Therefore, we evaluate the roles of microdialysis vs.1H-NMR as techniques to assess NAA (NAAd; NAA/Creatine ratio) in the living brain, and compare the results with whole brain NAA (NAAJ, analyzed by HPLC after diffuse traumatic brain injury (TBI). Acute (4 h post-injury survival) and late (48 h survival) changes were studied in a sham-operated group (Sham, n = 4), and two injured groups (TBI/4 h, n = 8; TBI/48 h, n = 7). Baseline NAAdwas 8.17±1 iM, and there was no significant difference between groups. There was only a small (twice of control), but transient increase in NAAdin the TBI/4 h group after trauma. Baseline NAA/Cr ratio was 1.35± 0.2, which did not change significantly between baseline, 1, 2, 3, 4 and 48 h or between groups after TBI. Whole brain NAAW(baseline 8.5± 0.5 mmol kg~ wet weight) did not differ significantly between groups before and after TBI. Diffuse TBI did not produce long-term changes in NAA, assessed by three different methods. These results may indicate that NAA is not a sensitive marker of the severity of diffuse axonal damage. However, further studies are needed to evaluate whether confounding factors such as microdialysis probe, voxel position and non-regional tissue homogenization might have influenced our data. [Neurol Res 2000;22:705-712]
