Endurance training attenuates the decrease in skeletal muscle malonyl-CoA with exercise
Endurance training attenuates the decrease in skeletal muscle malonyl-CoA with exercise
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DOI：
10.1152/jappl.1997.83.6.1917
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发表时间：
1997-12-01
影响因子：
3.3
通讯作者：
Winder, WW
中科院分区：
文献类型：
--
作者：
Hutber, CA;Rasmussen, BB;Winder, WW
据推测，肌肉中的丙二酸单酰辅酶A通过抑制肉碱棕榈酰转移酶1来调节脂肪酸代谢。在未经训练的大鼠中，运动期间工作肌肉中的丙二酸单酰辅酶A会减少。已知耐力训练会增加肌肉对脂肪酸作为底物的依赖。本研究旨在探讨在运动时，经过训练的肌肉中丙二酸单酰辅酶A的下降是否会比未经训练的肌肉更显著，从而使脂肪酸氧化增加。经过6 - 10周的耐力训练（每天2小时）或跑步机适应（每天5 - 10分钟）后，大鼠在休息时或在以21米/分钟的速度在15%坡度上跑5、20或60分钟后被处死。训练减弱了运动诱导的丙二酸单酰辅酶A在大鼠股四头肌红肌中的下降，并阻止了运动诱导的乙酰辅酶A羧化酶柠檬酸激活常数在运动20和60分钟时的增加。因此，与预期相反，在一次长时间的次最大强度运动中，经过训练的肌肉中丙二酸单酰辅酶A的减少量比未经训练的肌肉要少。
Muscle malonyl-CoA has been postulated to regulate fatty acid metabolism by inhibiting carnitine palmitoyltransferase 1. In nontrained rats, malonyl-CoA decreases in working muscle during exercise. Endurance training is known to increase a muscle's reliance on fatty acids as a substrate. This study was designed to investigate whether the decline in malonyl-CoA with exercise would be greater in trained than in nontrained muscle, thereby allowing increased fatty acid oxidation. After 6-10 wk of endurance training (2 h/day) or treadmill habituation (5-10 min/day), rats were killed at rest or after running up a 15% grade at 21 m/min for 5, 20, or 60 min. Training attenuated the exercise-induced drop in malonyl-CoA and prevented the exercise-induced increase in the constant for citrate activation of acetyl-CoA carboxylase in the red quadriceps muscle of rats run for 20 and 60 min. Hence, contrary to expectations, the decrease in malonyl-CoA was less in trained than in nontrained muscle during a single bout of prolonged submaximal exercise.