Competitive use of root exudates by Bacillus amyloliquefaciens with Ralstonia solanacearum decreases the pathogenic population density and effectively controls tomato bacterial wilt.
Competitive use of root exudates by Bacillus amyloliquefaciens with Ralstonia solanacearum decreases the pathogenic population density and effectively controls tomato bacterial wilt.
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DOI：
10.1016/j.scienta.2017.01.047
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发表时间：
2017-04
影响因子：
4.3
通讯作者：
Kai Wu;Lv Su;Zhiying Fang;Saifei Yuan;Lili Wang;Biao Shen;Q. Shen
中科院分区：
文献类型：
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作者：
Kai Wu;Lv Su;Zhiying Fang;Saifei Yuan;Lili Wang;Biao Shen;Q. Shen
了解有益细菌、植物病原菌和根分泌物之间的相互作用对于优化针对植物土传疾病的生物防治策略是必要的。在本研究中，我们发现拮抗细菌SQYUV 162和致病菌株青枯雷尔氏菌竞争利用根分泌物影响了病原菌的种群密度和致病力。青枯草。所有供试的有机酸都能诱导SQYUV 162和SQYUV 162产生趋化作用。青枯草。此外，SQYUV 162和DR.添加有机酸对青枯菌有一定的影响。在SQYUV 162中有强烈的趋化反应。分别用苹果酸和柠檬酸诱导青枯病。还观察到，添加有机酸显著诱导了几个与毒力相关的基因的表达，并增加了病原菌在根上的种群密度，导致水培试验中的发病率高于对照。然而，接种SQYUV 162后，致病相关基因的表达和病原菌的种群密度都降低了。当根与有机酸接触时，SQYUV 162的种群密度也增加。提高了SQYUV 162的定殖密度，降低了病原菌的致病性和种群密度，有效地抑制了青枯病的发生。目前的结果表明，根分泌物中的某些成分可以抑制土传病害：通过使用资源消耗谱与植物病原菌重叠的细菌，可以更好地控制植物病害。
Understanding the interactions between beneficial bacteria, plant pathogens, and root exudates is necessary for the optimization of the bio-control strategies targeted toward plant soil-borne diseases. In this study, we showed that the competitive use of root exudates by the antagonistic bacterial strain SQYUV 162 along with the pathogenic strainRalstonia solanacearumaffected the pathogenic population density and the pathogenicity ofR. solanacearum. All of the tested organic acids induced chemotaxis in both SQYUV 162 andR. solanacearum. In addition, the growth of both SQYUV 162 andR. solanacearumwas affected by the addition of organic acids. Strong chemotactic responses in SQYUV 162 andR. solanacearumwere induced by malic acid and citric acid respectively. It was also observed that the addition of organic acids significantly induced the expression of several virulence-related genes and increased the population density of the pathogen on the roots, resulting in higher disease incidences in hydroponic experiments in comparison to a control. When SQYUV 162 was inoculated, however, both the expression of virulence-related genes and the population density of the pathogen decreased. The population density of SQYUV 162 also increased when roots were placed in contact with organic acids. The improved colonization density of SQYUV 162, as well as the decreased pathogenicity and population density of the pathogen, efficiently suppressed the incidence of bacterial wilt. The present results indicate that certain components of root exudates could suppress soil-borne diseases: by applying bacteria whose resource consumption profile overlaps that of plant pathogens, plant diseases may be better controlled.