Candida albicans Zcf37, a zinc finger protein, is required for stabilization of the white state
Candida albicans Zcf37, a zinc finger protein, is required for stabilization of the white state
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白色念珠菌 Zcf37 是一种锌指蛋白，是稳定白色状态所必需的
DOI：
10.1016/j.febslet.2011.02.005
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发表时间：
2011-03-09
期刊：
影响因子：
3.5
通讯作者：
Chen, Jiangye
中科院分区：
文献类型：
--
作者：
Wang, Huafeng;Song, Wenji;Chen, Jiangye
白色念珠菌是最常见的人类真菌病原体，可以在白色和不透明相之间随机转换。在这项研究中，我们确定Zcf37，锌指蛋白，作为一个新的调节器的白色不透明开关。ZCF 37的缺失增加了白色到不透明的转换频率，并稳定了不透明状态。ZCF 37的过表达促进不透明细胞向白色相的转化，但需要Efg 1的存在，Efg 1是维持白色状态所需的关键调节因子。EFG 1的缺失消除了异位表达的Zcf 37对不透明到白色转换的影响，而EFG 1的异位表达促进了不存在Zcf 37的白色细胞的形成。我们的研究结果表明，Zcf 37作为白色细胞形成的激活剂和不透明状态的抑制剂，并在Efg 1的上游发挥作用。(C)2011年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
Candida albicans, the most prevalent human fungal pathogen, can switch stochastically between white and opaque phases. In this study, we identified Zcf37, a zinc finger protein, as a new regulator of white-opaque switching. Deletion of ZCF37 increased white-to-opaque switching frequency and stabilized the opaque state. Overexpression of ZCF37 promoted conversion of opaque cells to white phase, but needed existence of Efg1, a key regulator required for maintenance of the white state. Deletion of EFG1 abolished the effect of ectopically expressed Zcf37 on opaque-to-white switching, whereas ectopic expression of EFG1 promoted white cell formation without presence of Zcf37. Our results suggest that Zcf37 acts as an activator of white cell formation and a repressor of opaque state and functions upstream of Efg1. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.