The Regenerated Bone Quality by Implantation of Octacalcium Phosphate Collagen Composites in a Canine Alveolar Cleft Model
The Regenerated Bone Quality by Implantation of Octacalcium Phosphate Collagen Composites in a Canine Alveolar Cleft Model
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DOI：
10.1597/12-096
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发表时间：
2014-07-01
期刊：
影响因子：
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通讯作者：
Echigo, Seishi
中科院分区：
文献类型：
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作者：
Matsui, Aritsune;Matsui, Keiko;Echigo, Seishi
目的：在大鼠颅骨缺损模型中，合成磷酸八钙和猪去端胶原复合材料比β-磷酸三钙胶原复合材料和羟基磷灰石胶原复合材料更显着增强骨再生。然而，长期稳定性和质量的磷酸八钙胶原（OCP/Col）复合材料衍生的再生骨，当植入犬牙槽突裂模型，尚未阐明。本研究探讨了OCP/Col再生骨的长期稳定性和质量。设计：将OCP/Col或胶原植入犬牙槽突裂模型（n = 6）。然后，在植入后10个月，对植入区域的骨再生进行了肉眼观察、放射学检查和组织学检查。此外，再生骨的三维定量图像进行了分析microcomputedtomography.Results：宏观上，OCP/Col治疗的牙槽骨明显增强，在OCP/Col植入区域的不透射线性与原始牙槽骨。组织学分析显示，该区域大部分为新生骨填充，并有少量植入的OCP/Col颗粒包裹其中;显微CT分析显示，OCP/Col组的新生骨体积大于胶原组。结论：OCP/Co复合材料植入体内后可引起骨组织的高度再生和结构重建，是一种良好的骨再生材料，可替代自体骨。
Objective: Synthetic octacalcium phosphate and porcine atelocollagen composites significantly enhanced bone regeneration more than beta-tricalcium phosphate collagen composite and hydroxyapatite collagen composite in a rat cranial defect model. However, the long-term stability and quality of octacalcium phosphate collagen (OCP/Col) composites-derived regenerated bone, when implanted in a canine alveolar cleft model, have yet to be elucidated. The present study investigated the long-term stability and quality of bone regenerated by OCP/Col.Design: Disks of OCP/Col or collagen were implanted in a canine alveolar-cleft model (n = 6). Then, bone regeneration in the implanted areas was investigated macroscopically, radiographically, and histologically at 10 months after implantation. In addition, three-dimensional quantitative images of regenerated bone were analyzed by microcomputed tomography.Results: Macroscopically, the OCP/Col treated alveolus was clearly augmented, and radioopacity in the OCP/Col implanted area was comparable to that of the original alveolus bone. On histological analysis, the area was mostly filled with newly formed bone, and a few granules of implanted OCP/Col were enclosed in it. In the microcomputed tomography analysis, the regenerated bone volume in the OCP/Col group was larger than that in the collagen group. OCP/ Col-derived bone consisted of outer cortical and inner cancellous structure with dense trabeculae and seemed like the original bone structure.Conclusions: OCP/Co composites could be a useful bone regenerative material to substitute for autogenous bone because their implantation could elicit high bone regeneration and active structural reconstitution.