Defining the transcriptional redundancy of early bodywall muscle development in C-elegans:: evidence for a unified theory of animal muscle development
Defining the transcriptional redundancy of early bodywall muscle development in C-elegans:: evidence for a unified theory of animal muscle development
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DOI：
10.1101/gad.1481706
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发表时间：
2006-12-15
影响因子：
10.5
通讯作者：
Krause, Michael
中科院分区：
文献类型：
--
作者：
Fukushige, Tetsunari;Brodigan, Thomas M.;Krause, Michael
成肌调节因子（MRF）是哺乳动物骨骼肌发生所必需的。相反，体壁肌肉是很容易检测到秀丽隐杆线虫胚胎缺乏活动的孤独MRF直系同源HLH-1，表明额外的生肌因子必须在线虫的功能。我们发现两个额外的C。Elegans蛋白质，HND-120/SRF和HND-1/HAND，当在胚胎中异位过度产生时，可以将幼稚卵裂球转化为肌肉。此外，我们已经使用遗传无效突变体来证明这两种因子与HLH-1一起调节肌生成。所有三个因子的缺失导致胚胎缺乏可检测的体壁肌肉分化，将这三个因子确定为C.优雅在哺乳动物中，SRF和HAND在调节平滑肌和心肌发育中发挥重要作用。梭秀丽线虫体壁肌肉的发育依赖于与所有三种类型的哺乳动物肌肉相关的转录因子，这支持了所有动物肌肉类型都源自共同的祖先收缩细胞类型的理论。
Myogenic regulatory factors (MRFs) are required for mammalian skeletal myogenesis. In contrast, bodywall muscle is readily detectable in Caenorhabditis elegans embryos lacking activity of the lone MRF ortholog HLH-1, indicating that additional myogenic factors must function in the nematode. We find that two additional C. elegans proteins, UNC-120/SRF and HND-1/HAND, can convert naive blastomeres to muscle when overproduced ectopically in the embryo. In addition, we have used genetic null mutants to demonstrate that both of these factors act in concert with HLH-1 to regulate myogenesis. Loss of all three factors results in embryos that lack detectable bodywall muscle differentiation, identifying this trio as a set that is both necessary and sufficient for bodywall myogenesis in C. elegans. In mammals, SRF and HAND play prominent roles in regulating smooth and cardiac muscle development. That C. elegans bodywall muscle development is dependent on transcription factors that are associated with all three types of mammalian muscle supports a theory that all animal muscle types are derived from a common ancestral contractile cell type.