Non-homologous chromosome pairing and crossover formation in haploid rice meiosis
Non-homologous chromosome pairing and crossover formation in haploid rice meiosis
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单倍体水稻减数分裂中的非同源染色体配对和交叉形成
DOI：
10.1007/s00412-010-0288-3
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发表时间：
2011-02-01
期刊：
影响因子：
1.6
通讯作者：
Gu, Minghong
中科院分区：
文献类型：
--
作者：
Gong, Zhiyun;Liu, Xiuxiu;Gu, Minghong
虽然许多研究提供了显着的见解同源配对减数分裂期间，非同源配对的信息少得多。本研究利用荧光原位杂交技术（FISH）研究了单倍体水稻减数分裂过程中的非同源配对。在粗线期，非同源染色体配对形成联会复合体。FISH分析结果表明，染色体配对可分为3种主要类型：（1）单染色体配对折叠为单价体结构;（2）两条非同源染色体配对为二价体结构;（3）三条以上非同源染色体配对为多价体结构。在对70个细胞的调查中，65个含有单价体，45个含有二价体，49个含有多价体。此外，9号和10号染色体以及11号和12号染色体以高于其他染色体的频率形成非同源二价体。然而，在终变期或中期I，只有11和12号染色体之间的二价体中检测到交叉，表明这两条染色体之间的配对导致减数分裂期间的非同源重组。通过对RCE 8、PAIR 2和ZEP 1的免疫检测，进一步证明了非同源物之间的联会复合体形成。特别是在粗线期，ZEP 1只装载在配对染色体上，而不装载在非配对染色体上。
While many studies have provided significant insight into homolog pairing during meiosis, information on non-homologous pairing is much less abundant. In the present study, fluorescencein situhybridization (FISH) was used to investigate non-homologous pairing in haploid rice during meiosis. At pachytene, non-homologous chromosomes paired and formed synaptonemal complexes. FISH analysis data indicated that chromosome pairing could be grouped into three major types: (1) single chromosome paired fold-back as the univalent structure, (2) two non-homologous chromosomes paired as the bivalent structure, and (3) three or more non-homologous chromosomes paired as the multivalent structure. In the survey of 70 cells, 65 contained univalents, 45 contained bivalents, and 49 contained multivalent. Moreover, chromosomes 9 and 10 as well as chromosomes 11 and 12 formed non-homologous bivalents at a higher frequency than the other chromosomes. However, chiasma was always detected in the bivalent only between chromosomes 11 and 12 at diakinesis or metaphase I, indicating the pairing between these two chromosomes leads non-homologous recombination during meiosis. The synaptonemal complex formation between non-homologs was further proved by immunodetection of RCE8, PAIR2, and ZEP1. Especially, ZEP1 only loaded onto the paired chromosomes other than the un-paired chromosomes at pachytene in haploid.