Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis.
Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis.
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DOI：
10.1007/978-1-0716-1904-9_1
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发表时间：
2022-01-01
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影响因子：
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通讯作者：
Shuster, Charles B
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文献类型：
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作者：
Pal, Debadrita;Visconti, Florencia;Shuster, Charles B
自十九世纪以来，海胆和其他棘皮动物的早期胚胎一直作为研究细胞分裂的实验模型。它们的快速发展、光学清晰度和易于操作继续为研究纺锤体组装和胞质分裂提供优势。在没有转基因系的情况下，必须采用替代策略来可视化微管和肌动蛋白。在这里，我们描述了使用纯化的重组蛋白或体外转录 mRNA 中的探针来可视化肌动蛋白和微管的方法。
The early embryos of sea urchins and other echinoderms have served as experimental models for the study of cell division since the nineteenth century. Their rapid development, optical clarity, and ease of manipulation continue to offer advantages for studying spindle assembly and cytokinesis. In the absence of transgenic lines, alternative strategies must be employed to visualize microtubules and actin. Here, we describe methods to visualize actin and microtubule using either purified, recombinant proteins, or probes in in vitro-transcribed mRNAs.