REPRODUCTION OF EOSINOPENIA OF ACUTE INFECTION BY PASSIVE TRANSFER OF A MATERIAL OBTAINED FROM INFLAMMATORY EXUDATE
REPRODUCTION OF EOSINOPENIA OF ACUTE INFECTION BY PASSIVE TRANSFER OF A MATERIAL OBTAINED FROM INFLAMMATORY EXUDATE
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DOI：
10.1128/iai.15.2.410-416.1977
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发表时间：
1977-01-01
影响因子：
3.1
通讯作者：
BASS, DA
中科院分区：
文献类型：
--
作者：
BASS, DA
在先前因旋毛虫病（由旋毛虫引起）致嗜酸性粒细胞增多的小鼠中进行了急性炎症的嗜酸性粒细胞减少效应的研究。从肺炎球菌脓肿中取出的渗出液中含有一种物质（嗜酸性粒细胞减少因子[EF]），当注射到嗜酸性粒细胞增多的小鼠体内时，这种物质能引起持续4-24小时的嗜酸性粒细胞减少。材料通过0.045 μ m。m过滤器，但被透析膜保留，能够保留大于约30,000 MW的蛋白质分子。EF可溶于7%的高氯酸，在Trasylol存在下不被肺炎球菌蛋白水解酶破坏，但在56度加热时失活。C 30分钟。10 h后可在渗出液中检测到EF， 20 h后达到最高浓度。当EF的作用以抑制对照嗜酸性粒细胞水平的百分比表示时，存在几何剂量反应。红细胞减少不能归因于制剂中存在的类固醇，EF对肾上腺切除的动物有效。同样处理过的热杀肺炎球菌、肺炎球菌培养滤液或正常血清均未引起嗜酸性粒细胞减少。急性感染的红细胞减少可能是急性炎症部位存在的物质的直接作用。
Studies of the eosinopenic effect of acute inflammation were conducted in mice previously rendered eosinophilic with trichinosis [caused by Trichinella spiralis]. Exudate removed from a pneumococcal abscess contained material (eosinopenic factor [EF]) capable of causing eosinopenia of 4-24th duration when injected i.p. into eosinophilic mice. The material passed through a 0.045 .mu.m filter, but was retained by a dialysis membrane capable of retaining protein molecules of greater than approximately 30,000 MW. EF was soluble in 7% perchloric acid, was not destroyed by pneumococcal proteolytic enzymes in the presence of Trasylol, but was inactivated by heating to 56.degree. C for 30 min. EF was detectable in the exudate after 10 h and had reached its highest concentration after 20 h. When the effect of EF was expressed as a percent suppression of control eosinophil levels there was a geometric dose response. Eosinopenia could not be ascribed to steroids present in the preparation and the EF was effective in adrenalectomized animals. Eosinopenia was not induced by transfer of similarly treated heat-killed pneumococci, pneumococcal culture filtrate or normal serum. The eosinopenia of acute infection may be the direct effect of a substance present at the site of acute inflammation.