THE COMPLETE DNA-SEQUENCE OF THE MITOCHONDRIAL GENOME OF PODOSPORA-ANSERINA
THE COMPLETE DNA-SEQUENCE OF THE MITOCHONDRIAL GENOME OF PODOSPORA-ANSERINA
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DOI：
10.1007/bf00334517
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发表时间：
1990-05-01
期刊：
影响因子：
2.5
通讯作者：
MATSUURA, ET
中科院分区：
文献类型：
--
作者：
CUMMINGS, DJ;MCNALLY, KL;MATSUURA, ET
本文报道了鹅柄孢菌（Podospora anserina）S小种线粒体基因组94，192 bp的全序列（1 kb = 103 bp）。还提供了A族特有的三个区域，使该基因组的大小达到100，314bp。小种s含有31个Ⅰ类内含子（A小种33个）和2个Ⅱ类内含子（A小种3个）。分析表明，I组内含子可以根据家族的二级结构和它们的开放阅读框架进行分类。所有鉴定的基因都是从同一条链转录的。除了缺乏ATP酶9外，柄孢霉基因组包含与其真菌对应物粗糙脉孢霉和构巢曲霉相同的基因。大约20%的基因组尚未被识别。几个切除扩增质粒的DNA序列研究表明，一个共同的特点是存在于两个末端的短重复序列与流行的GGCGCAAGCTC。
The complete 94,192 bp sequence of the mitochondrial genome from race s of Podospora anserina is presented (1 kb = 103 base pairs). Three regions unique to race A are also presented bringing the size of this genome to 100,314 bp. Race s contains 31 group I introns (33 in race A) and 2 group II introns (3 in race A). Analysis shows that the group I introns can be categorized according to families both with regard to secondary structure and their open reading frames. All identified genes are transcribed from the same strand. Except for the lack of ATPase 9, the Podospora genome contains the same genes as its fungal counterparts, Neurospora crassa and Aspergillus nidulans. About 20% of the genome has not yet been identified. DNA sequence studies of several excision-amplification plasmids demonstrate a common feature to be the presence of short repeated sequences at both termini with a prevalence of GGCGCAAGCTC.