SMALL RNAS OF ROUS-SARCOMA VIRUS - CHARACTERIZATION BY 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS AND FINGERPRINT ANALYSIS
SMALL RNAS OF ROUS-SARCOMA VIRUS - CHARACTERIZATION BY 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS AND FINGERPRINT ANALYSIS
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DOI：
10.1128/jvi.12.6.1226-1237.1973
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发表时间：
1973-01-01
影响因子：
5.4
通讯作者：
DAHLBERG, JE
中科院分区：
文献类型：
--
作者：
SAWYER, RC;DAHLBERG, JE
Approximately 15 to 20 different species of small (4 to 7S) RNAs have been purified by two-dimensional polyacrylamide gel electrophoresis of RNA isolated from virions of Schmidt-Ruppin D strain of Rous sarcoma virus. Each species of small RNA has been isolated free of 70SRNA; nine of them, including 5Sand 7SRNAs, were also found associated with the 70Sgenomic RNA. Most of the 4SRNAs are present at an average of less than one copy per virion. The 4SRNAs have T1 RNase (EC 2.7.7.26) fingerprints, which are very similar to those of tRNAs. One of the smallest 4SRNAs, which can act as a primer for initiation of RNA-directed DNA synthesis, is associated with the 70SRNA in 1 to 2 copies per complex, whereas an additional 6 to 8 copies of this molecule are free.
