Fabrication of Amine-functionalized Acrylic Monoliths via Thermally Induced Phase Separation and Their Application for Separation Media
Fabrication of Amine-functionalized Acrylic Monoliths via Thermally Induced Phase Separation and Their Application for Separation Media
复制标题
通过热诱导相分离制备胺官能化丙烯酸整体材料及其在分离介质中的应用
DOI：
10.1007/s10934-016-0256-2
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发表时间：
2017
影响因子：
2.6
通讯作者：
H. Uyama
中科院分区：
文献类型：
--
作者：
T. Tsujimoto;T. Kitagawa;S. Yoneda;H. Uyama
本文研究了胺基丙烯酸酯单体的制备及其在分离介质中的应用。采用共溶剂体系通过热诱导相分离(TIPS)法制备了具有三维连通孔结构的聚甲基丙烯酸缩水甘油酯-甲基丙烯酸甲酯(PGMA)单体，然后环氧乙烷的氨解反应生成了胺功能化的丙烯酸酯单体。单体的孔结构和比表面积依赖于PGMA的相对分子质量。通过静电相互作用，牛血清白蛋白(BSA)被有效地吸附在整体柱上，结合能力随着整体柱比表面积的增大而增强。此外，胺功能化整体柱通过高盐溶液洗涤释放出牛血清白蛋白。这些结果表明，TIPS法制备胺功能化整体柱具有快速分离和分析生物分子的潜力。
This study deals with fabrication of amine-functionalized acrylic monoliths and their application for separation media. Poly(glycidyl methacrylate-co-methyl methacrylate) (PGMA) monoliths with three-dimensional interconnected porous structure were prepared by thermally induced phase separation (TIPS) using cosolvent system, and subsequent aminolysis of oxirane groups produced amine-functionalized acrylic monoliths. The porous structure and specific surface area of the monoliths were dependent on the molecular weight of PGMA. Bovine serum albumin (BSA) was effectively adsorbed on the monoliths by electrostatic interaction, and the binding capacity was enhanced with an increase in the surface area of the monoliths. Furthermore, the amine-functionalized monoliths released BSA by washing with high salt concentration solution. These results demonstrated a potential of the amine-functionalized monoliths prepared by TIPS method for rapid separation and analysis of biomolecules.