ERG, A HUMAN ETS-RELATED GENE ON CHROMOSOME-21 - ALTERNATIVE SPLICING, POLYADENYLATION, AND TRANSLATION
ERG, A HUMAN ETS-RELATED GENE ON CHROMOSOME-21 - ALTERNATIVE SPLICING, POLYADENYLATION, AND TRANSLATION
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DOI：
10.1126/science.3299708
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发表时间：
1987-08-07
期刊：
影响因子：
56.9
通讯作者：
REDDY, ESP
中科院分区：
文献类型：
--
作者：
RAO, VN;PAPAS, TS;REDDY, ESP
禽急性白血病病毒E26可诱导鸡红系和髓系混合白血病，并携带v-myb和v-Ets两个不同的癌基因。最近，在人Colo 320细胞中发现了一个与v-ETS癌基因密切相关的新基因erg，并测定了其约5.0kb转录本erg1的核苷酸序列。在本研究中，选择性剪接转录本erg2的核苷酸序列与erg1的不同之处在于剪接事件导致在氨基末端附近的编码移码，导致在氨基末端额外插入99个氨基酸。这两个转录本的互补DNA在体外表达导致了大约41千和52千道尔顿的多肽的合成，这表明在ERG蛋白中使用了替代的翻译起始密码子。体细胞遗传学分析将erg基因定位于人21号染色体。有人认为，剪接和聚腺苷酸化的选择性位点，以及翻译起始的选择性位点，允许从单个erg基因转录单位合成两个相关的多肽。
The avian acute leukemia virus E26 induces a mixed erythroid-myeloid leukemia in chickens and carries two distinct oncogenes, v-myb and v-ets. Recently, a novel gene named erg, closely related to the v-ets oncogene, was identified in human COLO 320 cells and the nucleotide sequence of its approximately 5.0-kilobase transcript, erg 1 was determined. In the present study, the nucleotide sequence of the alternatively spliced transcript, erg 2, was found to differ from erg 1 by a splicing event that causes a coding frameshift near the amino terminus, resulting in an additional 99-amino acid insertion at the amino-terminus. Expression of complementary DNAs for the two transcripts in vitro resulted in synthesis of polypeptides of approximately 41 and 52 kilodaltons, suggesting the use of alternative translation initiation codons in the case of erg proteins. The erg gene was localized by somatic cell genetic analysis to human chromosome 21. It is proposed that alternative sites of splicing and polyadenylation, together with alternative sites of translation initiation, allow the synthesis of two related polypeptides from a single erg gene transcriptional unit.