BRASSINOSTEROID-INDUCED RICE LAMINA JOINT INCLINATION AND ITS RELATION TO INDOLE-3-ACETIC-ACID AND ETHYLENE
BRASSINOSTEROID-INDUCED RICE LAMINA JOINT INCLINATION AND ITS RELATION TO INDOLE-3-ACETIC-ACID AND ETHYLENE
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DOI：
10.1007/bf00029540
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发表时间：
1995-03-01
影响因子：
4.2
通讯作者：
CHEN, SK
中科院分区：
文献类型：
--
作者：
CAO, HP;CHEN, SK
利用从蜂蜡中分离的油菜素内酯（BR）研究了油菜素内酯诱导水稻（Oriza sativa L,.）叶片关节（RLJ）倾斜度及其与吲哚-3-乙酸（IAA）和乙烯的关系。BR对RLJ倾斜度的影响具有时间和浓度依赖性。黄化层比绿色层对BR更敏感。br诱导的倾角增加了层鲜重、总含水量、自由水含量、质子挤出量和乙烯产量，降低了结合水含量。叶片干重未发生变化。这种倾斜是由于椎板关节的近轴细胞相对于背侧细胞的扩张更大。这种响应是由BR和/或BR诱导的信号从叶鞘传递到叶片引起的。br诱导的RLJ倾斜和乙烯生成均受到ACC氧化酶抑制剂氯化钴（CoCl2）的抑制。br诱导的倾斜度远高于IAA，并被高浓度IAA转运抑制剂2,3,5-三碘苯甲酸（TIBA）所抑制。BR与IAA之间存在协同效应。这些结果表明，BR对RLJ倾角和髓鞘细胞扩张的影响可能是由于BR增加了膜层的水势和质子挤压所致。br诱导的RLJ倾斜可能与乙烯的作用有关，但可能与IAA无关。
Brassinosteroid (BR)-induced rice (Oriza sativa L,.) lamina joint (RLJ) inclination and its relationship to indole-3-acetic acid (IAA) and ethylene were investigated using BR isolated from beeswax. The effect of BR on RLJ inclination was time- and concentration-dependent. Etiolated lamina were more sensitive to BR than green lamina. The BR-induced inclination was accompanied by increased lamina fresh weight, total water content, free-water content, proton extrusion and ethylene production, and decreased bound-water content. Lamina dry weight was not changed. The inclination was due to greater expansion of the adaxial cells relative to the dorsal cells in the lamina joint. This response was caused by BR and/or BR-induced signal(s) that were transported from the leaf sheath to the leaf blade. Both BR-induced RLJ inclination and ethylene production were inhibited by cobalt chloride (CoCl2), an inhibitor of ACC oxidase. BR-induced inclination was much higher than that of IAA, and was inhibited by high concentration of 2,3,5-triiodobenzoic acid (TIBA), an inhibitor of IAA transport. A synergistic effect was observed between BR and IAA. These results suggest that the effects of BR on RLJ inclination and pulvinus cell expansion may be resulted from BR-increased water potential and proton extrusion in the lamina. The BR-induced RLJ inclination may involve the action of ethylene but may be independent of IAA.