MORPHOLOGY OF MINERAL-DEPOSITS ON ENCRUSTED URINARY CATHETERS INVESTIGATED BY SCANNING ELECTRON-MICROSCOPY
MORPHOLOGY OF MINERAL-DEPOSITS ON ENCRUSTED URINARY CATHETERS INVESTIGATED BY SCANNING ELECTRON-MICROSCOPY
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DOI：
10.1016/s0022-5347(17)39095-x
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发表时间：
1989-11-01
影响因子：
6.6
通讯作者：
HUKINS, DWL
中科院分区：
文献类型：
--
作者：
COX, AJ;HUKINS, DWL
人工尿液中的鸟粪石和羟基磷灰石通过控制尿素酶的添加沉淀到导管材料表面。它们可以一起沉淀，也可以分开沉淀（通过省略人工尿液的成分），可以加入白蛋白，也可以不加入白蛋白（目的是模仿感染尿液中的蛋白碎片）。用x射线粉末衍射鉴定析出物，用扫描电镜检查人工包覆表面。在蛋白质的存在下，羟基磷灰石以一种不良结晶形式沉淀，聚集形成外壳。鸟粪石晶体具有较大的尺寸和独特的外观，在扫描电镜下很容易识别。15个病人的结痂导管也通过扫描电子显微镜检查，另外6个使用x射线微量分析。它们的外观与体外包覆的材料非常相似。结壳包括羟基磷灰石的形成和鸟粪石晶体的生长，与细菌密切相关。
Struvite and hydroxyapatite were precipitated from artificial urine onto the surfaces of catheter materials by the controlled addition of urease. They were precipitated both together and separately (by omitting components of the artificial urine), and with and without the inclusion of albumin (which was intended to mimic the proteinaceous debris found in infected urine). Precipitates were identified by X-ray powder diffraction and the artificially encrusted surfaces examined by scanning electron microscopy. In the presence of protein, hydroxyapatite was precipitated as a poorly crystalline form which aggregated to form a crust. Struvite crystals could be easily identified under the scanning electron microscope by their relatively large size and characteristic appearance. Fifteen encrusted catheters from patients were also examined by scanning electron microscopy, and a further six using X-ray microanalysis. Their appearance was very similar to that of the materials encrusted in vitro. Encrustation involves the formation of hydroxyapatite and the growth of struvite crystals, intimately associated with bacteria.