Genetics and molecular mapping of genes for high-temperature resistance to stripe rust in wheat cultivar Xiaoyan 54
Genetics and molecular mapping of genes for high-temperature resistance to stripe rust in wheat cultivar Xiaoyan 54
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DOI：
10.1007/s00122-011-1595-7
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发表时间：
2011-04
影响因子：
5.4
通讯作者：
X. L. Zhou;W. Wang;L. Wang;D. Hou;J. Jing;Y. Wang;Z. Q. Xu;Q. Yao;J. L. Yin;D. Ma
中科院分区：
文献类型：
--
作者：
X. L. Zhou;W. Wang;L. Wang;D. Hou;J. Jing;Y. Wang;Z. Q. Xu;Q. Yao;J. L. Yin;D. Ma
条锈病，由条锈菌引起。小麦赤霉病是世界范围内最广泛和最具破坏性的小麦病害之一。培育抗病品种是防治该病的首选手段。小麦品种小偃54具有高温抗条锈性。为了鉴定抗条锈病基因，以小偃54为母本，明贤169为父本，进行了抗条锈病基因的杂交。以中国小种CYR32为试材，在温室和大田条件下对亲本及F1、F2、F3、F4代的苗期和成株进行了测定。小偃54具有2对隐性抗高温基因，命名为Yrxy1和Yrxy2。利用SSR引物对一个F3分离系的181个单株进行了Yrxy2侧翼分子标记的筛选。共鉴定出9个标记，其中2个位于2A染色体长臂上遗传距离为4.0和6.4 cM的位点两侧。利用抗病基因类似物多态性（RGAP）和SSR技术鉴定与Yrxy1连锁的分子标记。利用另一分离系F3的177个单株构建了一个由9个RGAP标记和2个SSR标记组成的Yrxy 1连锁群。两个RGAP标记与该位点紧密连锁，遗传距离分别为2.3和3.5 cM。利用RGAP标记M8和M9以及位于7A染色体短臂上的SSR标记Yrxy 1对中国春缺四体材料进行扩增。SSR标记Xbarc49和Xwmc422分别距该基因15.8和26.1cM。这些紧密连锁的分子标记可用于将抗条锈基因整合到商品品种中，并与其他抗条锈基因进行组合。
Stripe rust, caused byPuccinia striiformisf. sp.tritici, is one of the most widespread and destructive wheat diseases worldwide. Growing resistant cultivars is the preferred means of control of the disease. The winter wheat cultivar Xiaoyan 54 has high-temperature resistance to stripe rust. To identify genes for stripe rust resistance, Xiaoyan 54 was crossed with Mingxian 169, a winter wheat genotype susceptible to all Chinese races of the pathogen. Seedlings and adult plants of the parents and F1, F2, F3and F4progeny were tested with Chinese race CYR32 under controlled greenhouse conditions and in the field. Xiaoyan 54 has two recessive resistance genes, designated asYrxy1andYrxy2, conferring high-temperature resistance. Simple sequence repeat (SSR) primers were used to identify molecular markers flankingYrxy2using 181 plants from one segregating F3line. A total of nine markers, two of which flanked the locus at genetic distances of 4.0 and 6.4 cM on the long arm of chromosome 2A were identified. Resistance gene analog polymorphism (RGAP) and SSR techniques were used to identify molecular markers linked toYrxy1. A linkage group of nine RGAP and two SSR markers was constructed forYrxy1using 177 plants of another segregating F3line. Two RGAP markers were closely linked to the locus with genetic distances of 2.3 and 3.5 cM. Amplification of a set of nulli-tetrasomic Chinese Spring lines with RGAP markersM8andM9and the two SSR markers locatedYrxy1on the short arm of chromosome 7A. The SSR markersXbarc49andXwmc422were 15.8 and 26.1 cM, respectively, from the gene. The closely linked molecular markers should be useful for incorporating the resistance genes into commercial cultivars and combining them with other genes for stripe rust resistance.