Interaction of human serum and neutrophils with Escherichia coli strains: differences between strains isolated from urine of patients with pyelonephritis or asymptomatic bacteriuria
Interaction of human serum and neutrophils with Escherichia coli strains: differences between strains isolated from urine of patients with pyelonephritis or asymptomatic bacteriuria
复制标题
人血清和中性粒细胞与大肠杆菌菌株的相互作用：从肾盂肾炎或无症状菌尿患者尿液中分离出的菌株之间的差异
作者：
B. Björkstén;B. Kaijser
从肾盂肾炎（n = 18）或无症状菌尿（ABU）（n = 20）的儿童尿液中分离的大肠杆菌菌株进行血清分型，并比较调理素的要求，对血清的敏感性，正常中性粒细胞在吞噬各种菌株过程中产生的化学发光量，以及荚膜抗原量。从ABU病例中分离的菌株比引起肾盂肾炎的菌株更能激活补体旁路途径。ABU菌株在新鲜的人血清中也有更好的调理作用，并且比肾盂肾炎菌株更经常血清敏感。在吞噬过程中的各种菌株的中性粒细胞化学发光有个别的变化，但两组菌株没有显着差异。来自肾盂肾炎病例的菌株比ABU菌株产生更大量的荚膜抗原。特定O或K抗原的存在与激活补体旁路途径的能力之间没有相关性。从ABU患者分离的菌株与从肾盂肾炎患者分离的菌株之间观察到的差异可能与进一步研究细菌毒力或宿主防御因子的差异有关。
Strains of Escherichia coli isolated from the urine of children with pyelonephritis (n = 18) or asymptomatic bacteriuria (ABU) (n = 20) were serotyped and compared with respect to opsonic requirements, sensitivity to serum, amount of chemiluminescence produced by normal neutrophils during phagocytosis of the various strains, and amount of capsular antigen. Strains isolated from cases of ABU more often had the capacity to activate the alternative complement pathway than had strains causing pyelonephritis. The ABU strains were also better opsonized in fresh human serum and were more often serum sensitive than the pyelonephritic strains. There were individual variations in neutrophil chemiluminescence during phagocytosis of the various strains, but the two groups of strains did not differ significantly. The strains from pyelonephritis cases produced a greater amount of capsular antigens than the ABU strains. There was no correlation between presence of a particular O or K antigen and capacity to activate the alternative complement pathway. The observed differences between strains isolated from patients with ABU and those isolated from patients with pyelonephritis may be relevant for further studies on differences in bacterial virulence or host defense factors.