Real-time non-invasive in vivo visible light detection of cortical spreading depolarizations in mice.
Real-time non-invasive in vivo visible light detection of cortical spreading depolarizations in mice.
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DOI：
10.1016/j.jneumeth.2018.09.001
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发表时间：
2018-11-01
影响因子：
3
通讯作者：
Ayata C
中科院分区：
文献类型：
--
作者：
Chung DY;Sugimoto K;Fischer P;Böhm M;Takizawa T;Sadeghian H;Morais A;Harriott A;Oka F;Qin T;Henninger N;Yaseen MA;Sakadžić S;Ayata C
皮质扩散去极化（CSD）是一种与偏头痛先兆相关的典型现象。CSD也与缺血性卒中、脑内出血、蛛网膜下腔出血和创伤性脑损伤后的继发性损伤有关;然而，大多数涉及这些疾病过程的研究都不能解释CSD的发生。在实验模型中检测CSD的一个主要障碍是，目前经过验证的方法是侵入性的，需要专门的设备和高水平的专业知识才能实施。我们提出了一种低成本，易于实施的方法来检测CSD在小鼠通过全层完整的头骨。我们的方法使用来自白色光照明（OIS-WL）的光学固有信号，并且允许使用容易获得的USB相机实时地在体内检测CSD。OIS-WL检测到100%的同时电极记录（28只小鼠中69个CSD）、激光多普勒血流仪（10只小鼠中82个CSD）、激光散斑血流仪（25只小鼠中68个CSD）或组合电极记录加激光散斑血流仪（20只小鼠中29个CSD）观察到的CSD。OIS-WL检测到激光多普勒血流仪遗漏的1个额外CSD。OIS-WL比电生理记录侵入性更小，比激光散斑血流测量更容易实现。此外，它提供了良好的空间和时间分辨率的动态成像的CSD在脑损伤的设置。利用廉价的USB摄像头和白色光源检测CSD为通过未改变的小鼠颅骨进行CSD的体内和非侵入性检测提供了可靠的方法。
Cortical spreading depolarization (CSD) is a phenomenon classically associated with migraine aura. CSDs have also been implicated in secondary injury following ischemic stroke, intracerebral hemorrhage, subarachnoid hemorrhage, and traumatic brain injury; however, most investigations involving these disease processes do not account for the occurrence of CSDs. A major barrier to detection of CSDs in experimental models is that currently validated methods are invasive and require specialized equipment and a high level of expertise to implement. We present a low-cost, easy-to-implement approach to the detection of CSDs in the mouse through full-thickness intact skull. Our method uses the optical intrinsic signal from white light illumination (OIS-WL) and allows for real-time in vivo detection of CSDs using readily available USB cameras. OIS-WL detected 100% of CSDs that were seen with simultaneous electrode recording (69 CSDs in 28 mice), laser Doppler flowmetry (82 CSDs in 10 mice), laser speckle flowmetry (68 CSDs in 25 mice), or combined electrode recording plus laser speckle flowmetry (29 CSDs in 20 mice). OIS-WL detected 1 additional CSD that was missed by laser Doppler flowmetry. OIS-WL is less invasive than electrophysiological recordings and easier to implement than laser speckle flowmetry. Moreover, it provides excellent spatial and temporal resolution for dynamic imaging of CSDs in the setting of brain injury. Detection of CSDs with an inexpensive USB camera and white light source provides a reliable method for the in vivo and non-invasive detection of CSDs through unaltered mouse skull.
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影响因子：
37.8
作者：
Eikermann-Haerter K;Lee JH;Yuzawa I;Liu CH;Zhou Z;Shin HK;Zheng Y;Qin T;Kurth T;Waeber C;Ferrari MD;van den Maagdenberg AM;Moskowitz MA;Ayata C
通讯作者：
Ayata C
影响因子：
4.9
作者：
Ayata, Cenk
通讯作者：
Ayata, Cenk
影响因子：
6.3
作者：
Shin, Hwa Kyoung;Dunn, Andrew K.;Ayata, Cenk
通讯作者：
Ayata, Cenk
DOI：
10.1097/00004647-200103000-00002
发表时间：
2001-03-01
影响因子：
6.3
作者：
Dunn, AK;Bolay, T;Boas, DA
通讯作者：
Boas, DA
影响因子：
3
作者：
Silasi, Gergely;Xiao, Dongsheng;Murphy, Timothy H.
通讯作者：
Murphy, Timothy H.