Silencing circular RNA hsa_circ_009755 promotes growth and metastasis of oral squamous cell carcinoma
Silencing circular RNA hsa_circ_009755 promotes growth and metastasis of oral squamous cell carcinoma
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沉默环状RNA hsa_circ_009755促进口腔鳞状细胞癌的生长和转移
DOI：
10.1016/j.ygeno.2020.09.035
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发表时间：
2020-11-01
期刊：
影响因子：
4.4
通讯作者：
Yang, Hongyu
中科院分区：
文献类型：
--
作者：
Zhang, Biru;Wang, Zihong;Yang, Hongyu
目的：口腔鳞状细胞癌(OSCC)是头颈部常见且恶性程度较高的恶性肿瘤。然而，其发病机制尚不清楚。本研究旨在探讨环状核糖核酸hsa_circ_009755对口腔鳞癌细胞增殖、迁移、侵袭和凋亡的影响。用实时定量聚合酶链式反应检测口腔鳞癌组织和细胞系中CircRNAhsa_CIRC_009755的表达。应用CCK-8、创伤愈合、Transwell和流式细胞仪分别检测口腔鳞癌细胞的增殖、迁移、侵袭和凋亡情况。结果：口腔鳞癌组织和3种口腔鳞癌细胞系中hsa_circ_009755低表达。结论：沉默hSA_CIRC_009755可能在口腔鳞癌的发生、发展中起重要作用。
Objective: Oral squamous cell carcinoma (OSCC) is a common and rampant malignancy of the head and neck. However, its pathogenesis remains unclear. In this study, we have investigated the effects of circular RNA hsa_circ_009755 on proliferation, migration, invasion, and apoptosis in OSCC cells in vitro.Method: Eight pairs of OSCC and normal adjacent tissues were selected to detect the differential expression of circRNAs by high-throughput sequencing. circRNA hsa_circ_009755 expression was determined by quantitative real-time polymerase chain reaction in OSCC tissues and cell lines. CCK-8, wound healing, Transwell, and flow cytometry assays were used to determine OSCC cell proliferation, migration, invasion, and apoptosis, respectively.Result: The expression of hsa_circ_009755 was low in OSCC tissues and three OSCC cell lines. Silencing hsa_circ_009755 significantly enhanced the proliferation, migration, and invasion and suppressed the apoptosis of OSCC cells.Conclusion: Therefore, hsa_circ_009755 may be important in the tumorigenesis of OSCC.