Transcriptional profiling of wheat caryopsis development using cDNA microarrays
Transcriptional profiling of wheat caryopsis development using cDNA microarrays
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DOI：
10.1007/s11103-006-9114-y
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发表时间：
2007-03-01
影响因子：
5.1
通讯作者：
Anderson, Olin D.
中科院分区：
文献类型：
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作者：
Laudencia-Chingcuanco, Debbie L.;Stamova, Boryana S.;Anderson, Olin D.
利用cDNA微阵列技术分析了7，835个基因在小麦颖果发育过程中的表达。使用混合模型方差分析（ANOVA）方法，鉴定出阵列上29%（2，237）的基因在检查的6个不同时间点差异表达，其涵盖从多核胚乳到生理成熟的发育阶段。两个时间点之间差异表达的基因的比较揭示了动态转录物积累概况，其中主要重编程事件发生在DPA 3-7、7-14和21-28。k-means聚类算法将差异表达的基因分成10个簇，揭示了参与相同途径的基因的共表达，例如碳水化合物和蛋白质合成或干燥的准备。在与相应阶段相关的发育事件相关的特定时间点显示峰值表达的基因的功能注释。结果提供了大量功能未知的差异表达基因在颖果发育过程中的时间表达信息。
The expression of 7,835 genes in developing wheat caryopses was analyzed using cDNA arrays. Using a mixed model analysis of variance (ANOVA) method, 29% (2,237) of the genes on the array were identified to be differentially expressed at the 6 different time-points examined, which covers the developmental stages from coenocytic endosperm to physiological maturity. Comparison of genes differentially expressed between two time-points revealed a dynamic transcript accumulation profile with major re-programming events that occur at 3-7, 7-14 and 21-28 DPA. A k-means clustering algorithm grouped the differentially expressed genes into 10 clusters, revealing co-expression of genes involved in the same pathway such as carbohydrate and protein synthesis or preparation for desiccation. Functional annotation of genes that show peak expression at specific time-points correlated with the developmental events associated with the respective stages. Results provide information on the temporal expression during caryopsis development for a significant number of differentially expressed genes with unknown function.